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Calcein UltraGreen™ AM
Calcein UltraGreen™ AM readily passes through the cell membrane of viable cells. Upon transporting into live cells cellular esterases cut off the AM groups, the molecule gets trapped inside cells. Compared with Calcein AM, Calcein UltraGreen™ is more suitable fluorescent probe for staining viable cells because of its lower cytotoxicity and longer retention in cells. UltraGreen™ AM does not significantly affect cellular functions such as proliferation or chemotaxis of lymophocyte.
Fixability of live HeLa cells stained with Calcein AM and Calcein UltraGreen AM. HeLa cells were stained with Calcein AM (Catalog #22004) and Calcein UltraGreen AM (Catalog #21905) and then fixed in 4% formaldehyde. Image acquisition was performed on a fluorescence microscope equipped with a FITC filter set. Notably, staining achieved with Calcein UltraGreen AM demonstrated prolonged stability post-fixation, indicating its effectiveness for long-term cellular imaging applications.
Fixability of live HeLa cells stained with Calcein AM and Calcein UltraGreen AM. HeLa cells were stained with Calcein AM (Catalog #22004) and Calcein UltraGreen AM (Catalog #21905) and then fixed in 4% formaldehyde. Image acquisition was performed on a fluorescence microscope equipped with a FITC filter set. Notably, staining achieved with Calcein UltraGreen AM demonstrated prolonged stability post-fixation, indicating its effectiveness for long-term cellular imaging applications.
CatalogSize
Price
Quantity
2190510x50 ug
Price
 
Physical properties

Molecular weight607.47
SolventDMSO
Spectral properties

Excitation (nm)492
Emission (nm)514
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation490
Emission525
Cutoff515
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on January 23, 2025