Calcein | AAT Bioquest

Calcein

UltraPure Grade; CAS 207124-64-9

Calcein, also known as fluorexon, fluorescein complex, is a fluorescent calcium indicator with an excitation and emission wavelengths of 495 and 515nm respectively. Calcein self-quenches at concentrations above 100 mM. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorimetric determination of calcium. It is slightly soluble in water. The proton dissociation constants are reported to be pKa1=2.1, pKa2=2.9, pKa3=4.2, pKa4=5.5, pKa5=10.8 and pKa6=11.7. Calcein solution emits a yellowish-green fluorescence in acidic conditions, and no fluorescence in basic conditions. However, calcein emits fluorescence in the presence of metal ions such as Al, Ba, Cu, Mg and Zn in basic conditions. Therefore, calcein can also be used for direct fluorimetric titration of these heavy metal ions besides Ca.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22001	10 mg	Price

Citations

View all 17 citations: Citation Explorer

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice

Authors:

Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang

Journal:

Biomedical Optics Express (2017): 2599--2610

NINJ2--A novel regulator of endothelial inflammation and activation

Authors:

Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined

Journal:

Cellular Signalling (2017)

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B

Authors:

Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres

Journal:

Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water

Authors:

Alharbi, Hattan A and Saunders, David MV and Al-Mousa, Ahmed and Alcorn, Jane and Pereira, Alberto S and Martin, Jonathan W and Giesy, John P and Wiseman, Steve B

Journal:

Aquatic Toxicology (2016): 81--88

Rapid generation of collagen-based microtissues to study cell--matrix interactions

Authors:

Brett, Marie-Elena and Crampton, Alex and ra L , undefined and Wood, David K

Journal:

Technology (2016): 1--8

References

View all 84 references: Citation Explorer

Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development

Authors:

Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang

Journal:

Biochemical and Biophysical Research Communications (2017)

Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine

Authors:

Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.

Journal:

Biophys J. (2006)

A vaccination and challenge model using calcein marked fish

Authors:

Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.

Journal:

Fish Shellfish Immunol (2006): 20

Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging

Authors:

Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.

Journal:

Cytometry A (2005): 78

Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake

Authors:

Schoonen WG, Westerink WM, de Roos JA, Debiton E.

Journal:

Toxicol In Vitro (2005): 505

Physical properties

Molecular weight	622.53
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	81000
Excitation (nm)	501
Emission (nm)	521

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	207124-64-9

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Page updated on January 23, 2025