Caliber Fluor™ 580

Caliber Fluor™ 580 is an extremely stable fluorophore that has its absorption and fluorescence independent of pH change from pH 3 to 10. It can be used to calibrate microplate wells and cuvettes. It can also be used for testing pipetting accuracy.

General packaging for catalog 95. Actual shipped packaging may vary.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
95	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Caliber Fluor™ 580 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	167.572 µL	837.858 µL	1.676 mL	8.379 mL	16.757 mL
5 mM	33.514 µL	167.572 µL	335.143 µL	1.676 mL	3.351 mL
10 mM	16.757 µL	83.786 µL	167.572 µL	837.858 µL	1.676 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	596.76 g/mol	=	mL	x	mM	=	mmol

References

View all 8 references: Citation Explorer

A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye

Authors:

Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.

Journal:

Am J Trop Med Hyg (2014): 306

A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9

Authors:

Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.

Journal:

Clin Lab (2013): 795

Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye

Authors:

Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.

Journal:

Mol Cell Probes (2012): 121

Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death

Authors:

Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.

Journal:

BMC Res Notes (2012): 437

SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR

Authors:

Eischeid AC., undefined

Journal:

BMC Res Notes (2011): 263

Physical properties

Molecular weight	596.76
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025