Name: CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers*
Brand: AAT Bioquest
SKU: 235
Price: 115 USD
Availability: InStock

CDCF

5-(and-6)-Carboxy-2',7'-dichlorofluorescein; Mixed isomers

CDCF [5(6)-carboxy-2',7'-dichlorofluorescein] is the fluorescent product of 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate and 5(6)-carboxy-2',7'-dichlorofluorescein diacetate in cells. The cell-permeant 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) (also known as dichlorofluorescin diacetate) is a chemically reduced form of fluorescein used as an indicator for reactive oxygen species (ROS) in cells, for example to detect the generation of reactive oxygen intermediates in neutrophils and macrophages. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the non-fluorescent H2DCFDA is converted to the highly fluorescent CDCF. 5(6)-carboxy-2',7'-dichlorofluorescein diacetate staining is used for studying multidrug resistance proteins [MRPs and P-glycoprotein (Pgp)].

Protocol

SDS

COA

Catalog	Size	Price	Quantity
235	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	224.613 µL	1.123 mL	2.246 mL	11.231 mL	22.461 mL
5 mM	44.923 µL	224.613 µL	449.226 µL	2.246 mL	4.492 mL
10 mM	22.461 µL	112.307 µL	224.613 µL	1.123 mL	2.246 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	445.21 g/mol	=	mL	x	mM	=	mmol

References

View all 23 references: Citation Explorer

Role of oxidative stress in the apoptosis of hepatocellular carcinoma induced by combination of arsenic trioxide and ascorbic acid

Authors:

Li JJ, Tang Q, Li Y, Hu BR, Ming ZY, Fu Q, Qian JQ, Xiang JZ.

Journal:

Acta Pharmacol Sin (2006): 1078

Effect of culture conditions on the expression and function of Bsep, Mrp2, and Mdr1a/b in sandwich-cultured rat hepatocytes

Authors:

Turncliff RZ, Tian X, Brouwer KL.

Journal:

Biochem Pharmacol (2006): 1520

Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress

Authors:

Shabala L, McMeekin T, Budde BB, Siegumfeldt H.

Journal:

Int J Food Microbiol (2006): 1

Kinetic validation of the use of carboxydichlorofluorescein as a drug surrogate for MRP5-mediated transport

Authors:

Pratt S, Chen V, Perry WI, 3rd, Starling JJ, Dantzig AH.

Journal:

Eur J Pharm Sci (2006): 524

Altered hepatobiliary disposition of 5 (and 6)-carboxy-2',7'-dichlorofluorescein in Abcg2 (Bcrp1) and Abcc2 (Mrp2) knockout mice

Authors:

Nezasa K, Tian X, Zamek-Gliszczynski MJ, Patel NJ, Raub TJ, Brouwer KL.

Journal:

Drug Metab Dispos (2006): 718

Physical properties

Molecular weight	445.21
Solvent	DMSO

Spectral properties

Excitation (nm)	505
Emission (nm)	526

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	111843-78-8

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Page updated on September 30, 2025