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CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers*

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Physical properties
Molecular weight445.21
SolventDMSO
Spectral properties
Excitation (nm)505
Emission (nm)526
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
111843-78-8
Molecular weight
445.21
Excitation (nm)
505
Emission (nm)
526
CDCF [5(6)-carboxy-2',7'-dichlorofluorescein] is the fluorescent product of 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate and 5(6)-carboxy-2',7'-dichlorofluorescein diacetate in cells. The cell-permeant 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) (also known as dichlorofluorescin diacetate) is a chemically reduced form of fluorescein used as an indicator for reactive oxygen species (ROS) in cells, for example to detect the generation of reactive oxygen intermediates in neutrophils and macrophages. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the non-fluorescent H2DCFDA is converted to the highly fluorescent CDCF. 5(6)-carboxy-2',7'-dichlorofluorescein diacetate staining is used for studying multidrug resistance proteins [MRPs and P-glycoprotein (Pgp)].

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM224.613 µL1.123 mL2.246 mL11.231 mL22.461 mL
5 mM44.923 µL224.613 µL449.226 µL2.246 mL4.492 mL
10 mM22.461 µL112.307 µL224.613 µL1.123 mL2.246 mL

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Spectrum


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spectrum

Spectral properties

Excitation (nm)505
Emission (nm)526

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Citations


View all 1 citations: Citation Explorer
Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress
Authors: Liu, Ying and Liu, Kejia and Thorne, Rick F and Shi, Ronghua and Zhang, Qingyuan and Wu, Mian and Liu, Lianxin
Journal: Cell Reports (2023)

References


View all 23 references: Citation Explorer
Role of oxidative stress in the apoptosis of hepatocellular carcinoma induced by combination of arsenic trioxide and ascorbic acid
Authors: Li JJ, Tang Q, Li Y, Hu BR, Ming ZY, Fu Q, Qian JQ, Xiang JZ.
Journal: Acta Pharmacol Sin (2006): 1078
Effect of culture conditions on the expression and function of Bsep, Mrp2, and Mdr1a/b in sandwich-cultured rat hepatocytes
Authors: Turncliff RZ, Tian X, Brouwer KL.
Journal: Biochem Pharmacol (2006): 1520
Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress
Authors: Shabala L, McMeekin T, Budde BB, Siegumfeldt H.
Journal: Int J Food Microbiol (2006): 1
Kinetic validation of the use of carboxydichlorofluorescein as a drug surrogate for MRP5-mediated transport
Authors: Pratt S, Chen V, Perry WI, 3rd, Starling JJ, Dantzig AH.
Journal: Eur J Pharm Sci (2006): 524
Altered hepatobiliary disposition of 5 (and 6)-carboxy-2',7'-dichlorofluorescein in Abcg2 (Bcrp1) and Abcc2 (Mrp2) knockout mice
Authors: Nezasa K, Tian X, Zamek-Gliszczynski MJ, Patel NJ, Raub TJ, Brouwer KL.
Journal: Drug Metab Dispos (2006): 718
CTLA-4IG suppresses reactive oxygen species by preventing synovial adherent cell-induced inactivation of Rap1, a Ras family GTPASE mediator of oxidative stress in rheumatoid arthritis T cells
Authors: Remans PH, Wijbr and ts CA, S and ers ME, Toes RE, Breedveld FC, Tak PP, van Laar JM, Reedquist KA.
Journal: Arthritis Rheum (2006): 3135
Non-destructive micromethod for MRP1 functional assay in human lung tumor cells
Authors: Stehfest E, Torky A, Glahn F, Foth H.
Journal: Arch Toxicol (2006): 125
Tracing the endocytic pathway of Aspergillus nidulans with FM4-64
Authors: Penalva MA., undefined
Journal: Fungal Genet Biol (2005): 963
Expression and activity of multidrug resistance protein 1 in a murine thymoma cell line
Authors: Echevarria-Lima J, Kyle-Cezar F, DF PL, Capella L, Capella MA, Rumjanek VM.
Journal: Immunology (2005): 468
Nitric oxide enhances catechol estrogen-induced oxidative stress in LNCaP cells
Authors: Muz, undefined and u K, Shaban Z, Ishizuka M, Kazusaka A, Fujita S.
Journal: Free Radic Res (2005): 389