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CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers*
CDCF [5(6)-carboxy-2',7'-dichlorofluorescein] is the fluorescent product of 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate and 5(6)-carboxy-2',7'-dichlorofluorescein diacetate in cells. The cell-permeant 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) (also known as dichlorofluorescin diacetate) is a chemically reduced form of fluorescein used as an indicator for reactive oxygen species (ROS) in cells, for example to detect the generation of reactive oxygen intermediates in neutrophils and macrophages. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the non-fluorescent H2DCFDA is converted to the highly fluorescent CDCF. 5(6)-carboxy-2',7'-dichlorofluorescein diacetate staining is used for studying multidrug resistance proteins [MRPs and P-glycoprotein (Pgp)].
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 224.613 µL | 1.123 mL | 2.246 mL | 11.231 mL | 22.461 mL |
| 5 mM | 44.923 µL | 224.613 µL | 449.226 µL | 2.246 mL | 4.492 mL |
| 10 mM | 22.461 µL | 112.307 µL | 224.613 µL | 1.123 mL | 2.246 mL |
Molarity calculator
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Spectrum
Citations
View all 1 citations: Citation Explorer
Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress
Authors: Liu, Ying and Liu, Kejia and Thorne, Rick F and Shi, Ronghua and Zhang, Qingyuan and Wu, Mian and Liu, Lianxin
Journal: Cell Reports (2023)
Authors: Liu, Ying and Liu, Kejia and Thorne, Rick F and Shi, Ronghua and Zhang, Qingyuan and Wu, Mian and Liu, Lianxin
Journal: Cell Reports (2023)
References
View all 23 references: Citation Explorer
Role of oxidative stress in the apoptosis of hepatocellular carcinoma induced by combination of arsenic trioxide and ascorbic acid
Authors: Li JJ, Tang Q, Li Y, Hu BR, Ming ZY, Fu Q, Qian JQ, Xiang JZ.
Journal: Acta Pharmacol Sin (2006): 1078
Authors: Li JJ, Tang Q, Li Y, Hu BR, Ming ZY, Fu Q, Qian JQ, Xiang JZ.
Journal: Acta Pharmacol Sin (2006): 1078
Effect of culture conditions on the expression and function of Bsep, Mrp2, and Mdr1a/b in sandwich-cultured rat hepatocytes
Authors: Turncliff RZ, Tian X, Brouwer KL.
Journal: Biochem Pharmacol (2006): 1520
Authors: Turncliff RZ, Tian X, Brouwer KL.
Journal: Biochem Pharmacol (2006): 1520
Listeria innocua and Lactobacillus delbrueckii subsp. bulgaricus employ different strategies to cope with acid stress
Authors: Shabala L, McMeekin T, Budde BB, Siegumfeldt H.
Journal: Int J Food Microbiol (2006): 1
Authors: Shabala L, McMeekin T, Budde BB, Siegumfeldt H.
Journal: Int J Food Microbiol (2006): 1
Kinetic validation of the use of carboxydichlorofluorescein as a drug surrogate for MRP5-mediated transport
Authors: Pratt S, Chen V, Perry WI, 3rd, Starling JJ, Dantzig AH.
Journal: Eur J Pharm Sci (2006): 524
Authors: Pratt S, Chen V, Perry WI, 3rd, Starling JJ, Dantzig AH.
Journal: Eur J Pharm Sci (2006): 524
Altered hepatobiliary disposition of 5 (and 6)-carboxy-2',7'-dichlorofluorescein in Abcg2 (Bcrp1) and Abcc2 (Mrp2) knockout mice
Authors: Nezasa K, Tian X, Zamek-Gliszczynski MJ, Patel NJ, Raub TJ, Brouwer KL.
Journal: Drug Metab Dispos (2006): 718
Authors: Nezasa K, Tian X, Zamek-Gliszczynski MJ, Patel NJ, Raub TJ, Brouwer KL.
Journal: Drug Metab Dispos (2006): 718
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