CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*

CDCFDA, SE

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester; Mixed isomers

It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample. Flow cytometry combined with fluorescent staining is a powerful tool to analyze heterogeneous cell populations. Fluorescein diacetate (FDA) and its derivatives are non-fluorescent molecules that diffuse into cells and are hydrolyzed by intracellular non-specific esterases to give fluorescent products. The fluorescent products can be accumulated only in those cells that have intact cell membranes; therefore, dead cells with leaky membranes are not stained. The precise kinetics of membrane transport and intracellular hydrolysis of FDA and its analogs (such as CDCFDA) are related to cellular functions, thus FDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The fluorescence intensity of labeled cells by FDA dyes varies considerably among cell lines and strains, probably because of differences in intracellular esterase activity. 5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester, is an amine-reactive FDA derivative that can be used to prepare a variety of FDA conjugates.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22026	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	159.655 µL	798.276 µL	1.597 mL	7.983 mL	15.966 mL
5 mM	31.931 µL	159.655 µL	319.31 µL	1.597 mL	3.193 mL
10 mM	15.966 µL	79.828 µL	159.655 µL	798.276 µL	1.597 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	626.35 g/mol	=	mL	x	mM	=	mmol

Citations

View all 9 citations: Citation Explorer

Periderm fate and independence of tooth formation are conserved across osteichthyans

Authors:

Huysseune, A and Horackova, A and Suchanek, T and Larionova, D and Cerny, R

Journal:

EvoDevo (2024): 13

Comparative development \& evolution of adenohypophysis in basal fishes

Authors:

Brezarov{\'a}, Dominika

Trabekula jako viscer{\'a}ln{\'\i} element p{\v{r}}edn{\'\i} {\v{c}}{\'a}sti hlavy obratlovc{\uu}

Authors:

Hor{\'a}{\v{c}}kov{\'a}, Ag{\'a}ta

Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish

Authors:

Oralov{\'a}, Veronika and Rosa, Joana T and Soenens, Mieke and Bek, Jan Willem and Willaert, Andy and Witten, Paul Eckhard and Huysseune, Ann

Journal:

Biology open (2019): bio042374

Periderm invasion contributes to epithelial formation in the teleost pharynx

Authors:

Rosa, Joana Teixeira and Oralová, Veronika and Larionova, Daria and Eisenhoffer, GT and Witten, P Eckhard and Huysseune, Ann

Journal:

Scientific reports (2019): 10082

References

View all 68 references: Citation Explorer

Novel method for cell debris removal in the flow cytometric cell cycle analysis using carboxy-fluorescein diacetate succinimidyl ester

Authors:

Terho P, Lassila O.

Journal:

Cytometry A (2006): 552

Modification of the fluorescein diacetate assay for screening of antifungal agents against Candida albicans: comparison with the NCCLS methods

Authors:

Brouwer N, Kohen J, Jamie J, Vemulpad S.

Journal:

J Microbiol Methods (2006): 234

Optimisation of the fluorescein diacetate antibacterial assay

Authors:

Wan, undefined and y S, Brouwer N, Liu Q, Mahon A, Cork S, Karuso P, Vemulpad S, Jamie J.

Journal:

J Microbiol Methods (2005): 21

A three-dimensional flow control concept for single-cell experiments on a microchip. 2. Fluorescein diacetate metabolism and calcium mobilization in a single yeast cell as stimulated by glucose and pH changes

Authors:

Peng XY, Li PC.

Journal:

Anal Chem (2004): 5282

Effect of immunosuppressants on T-cell subsets observed in vivo using carboxy-fluorescein diacetate succinimidyl ester labeling

Authors:

Hu H, Dong Y, Feng P, Fechner J, Hamawy M, Knechtle SJ.

Journal:

Transplantation (2003): 1075

Physical properties

Molecular weight	626.35
Solvent	DMSO

Spectral properties

Excitation (nm)	505
Emission (nm)	526

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on September 29, 2025