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Cell Explorer™ Live Cell Labeling Kit
Orange Fluorescence with 405 nm Excitation
Our Cell Explorer™ fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells for the flow cytometric analysis of live cells with the violet laser (405 nm excitation). The kit uses a proprietary dye that gets enhanced fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. It can be readily adapted for flow cytometry applications. The fluorescent dye used in the kit is well excited with the violet laser (405 nm excitation) to fluorescence at ~550 nm. The kit provides all the essential components with an optimized cell-labeling protocol. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.
Fluorescence images of HeLa cells stained with Cell Explorer&trade; Live Cell Labeling Kits <em>Orange Fluorescence with 405 nm Excitation</em>&nbsp;(Cat#22616) in a Costar black wall/clear bottom 96-well plate. Cells were stained with&nbsp;CytoCalcein&trade; Violet 550 for 30 minutes. Image was aquired using fluorescence microscope.
Fluorescence images of HeLa cells stained with Cell Explorer&trade; Live Cell Labeling Kits <em>Orange Fluorescence with 405 nm Excitation</em>&nbsp;(Cat#22616) in a Costar black wall/clear bottom 96-well plate. Cells were stained with&nbsp;CytoCalcein&trade; Violet 550 for 30 minutes. Image was aquired using fluorescence microscope.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22616200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission525/50 nm filter
Instrument specification(s)Pacific Orange channel
Fluorescence microscope
Excitation405 nm
Emission545/25 filter
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2025