Cell Explorer™ Live Cell Labeling Kit *Orange Fluorescence with 405 nm Excitation*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
Platform
Flow cytometer
Excitation | 405 nm laser |
Emission | 525/50 nm filter |
Instrument specification(s) | Pacific Orange channel |
Fluorescence microscope
Excitation | 405 nm |
Emission | 545/25 filter |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells in growth medium
- Remove growth medium
- Add CytoCalcein™ Violet 550 working solution (100 µL/well for a 96-well plate or 25 µL/well for a 384-well plate)
- Incubate the cells at 37°C for 30 minutes to 1 hour
- Wash the cells
- Examine the specimen under fluorescence microscope with 545/25 filter or flow cytometer with a 525/50 nm filter (Pacific Orange channel)
Important notes
Thaw all the components at room temperature before opening.
PREPARATION OF STOCK SOLUTION
1. CytoCalcein™ Violet 550 stock solution:
Add 20 µL of DMSO into the vial of CytoCalcein™ Violet 550 (Component A) and mix well to make CytoCalcein™ Violet 550 stock solution. Note: 20 µL of CytoCalcein™ Violet 550 stock solution is enough for 1 plate. Note: Unused CytoCalcein™ Violet 550 stock solution can be aliquoted and stored at < -20 oC for one month if the tubes are sealed tightly. Avoid repeated freeze-thaw cycles and protect it from light.
PREPARATION OF WORKING SOLUTION
Add 20 µL of CytoCalcein™ Violet 550 stock solution into 10 mL of HHBS (Component B) and mix well to make CytoCalcein™ Violet 550 working solution.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Remove the growth medium.
- Add 100 µL/well (96-well plate) or 25 µL/well (384-well plate) of CytoCalcein™ Violet 550 working solution into the cell plate.
- Incubate the cells in a 37°C, 5% CO2 incubator for 30 minutes to 1 hour.
- Remove the CytoCalcein™ Violet 550 working solution from the cells, wash the cells with HHBS (Component B) for 2 to 3 times, and replace with HHBS.
- Analyze the cells using a fluorescence microscope with 545/25 filter or flow cytometer with a 525/50 nm filter (Pacific Orange channel).
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Explorer™ Live Cell Labeling Kit *Blue Fluorescence with 405 nm Excitation* | 406 | 445 |
Cell Explorer™ Live Cell Labeling Kit *Green Fluorescence with 405 nm Excitation* | 420 | 505 |
Images

Citations
Authors: Cemma, Marija and Grinstein, Sergio and Brumell, John H
Journal: Autophagy (2016): 1440--1446
Authors: Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo
Journal: Analytical chemistry (2014): 6088--6094
Authors: Hu, Qing and Li, Yuli and Miao, Guohou and Zhao, Naru and Chen, Xiaofeng
Journal: Rsc Advances (2014): 22678--22687
Authors: Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan
Journal: Journal of Materials Chemistry (2012): 16906--16913
References
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Application notes
FAQ
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