Cell Explorer™ Live Cell Labeling Kit *Orange Fluorescence with 405 nm Excitation*

Cell Explorer™ Live Cell Labeling Kit

Orange Fluorescence with 405 nm Excitation

Cell Explorer™ Live Cell Labeling Kit is a fluorescence-based kit for labeling living cells with an esterase-activated orange fluorescent indicator compatible with 405 nm laser excitation.

Esterase-activated labeling: Intracellular esterases convert the weakly fluorescent substrate into a strongly fluorescent product
Cytoplasmic retention: Generates a hydrophilic fluorescent product that is retained in the cell cytoplasm
Violet-laser compatibility: Supports 405 nm excitation with fluorescence emission around 550 nm
Applications: Suitable for live-cell flow cytometry, cell adhesion and chemotaxis studies, and fluorescence-based cell assays

Fluorescence images of HeLa cells stained with Cell Explorer™ Live Cell Labeling Kits <em>Orange Fluorescence with 405 nm Excitation</em> (Cat#22616) in a Costar black wall/clear bottom 96-well plate. Cells were stained with CytoCalcein™ Violet 550 for 30 minutes. Image was aquired using fluorescence microscope.

Protocol

SDS

Catalog	Size	Price	Quantity
22616	200 Tests	Price

Overview

Cell Explorer™ Live Cell Labeling Kit is designed for orange fluorescent labeling of living cells using CytoCalcein™ Violet 550. The cell-permeable, weakly fluorescent substrate enters cells, where intracellular esterases hydrolyze it to produce a strongly fluorescent, hydrophilic product. Retention of this product in the cytoplasm enables fluorescence-based detection of labeled cells.

The kit includes CytoCalcein™ Violet 550 and HHBS buffer, with sufficient reagents for 200 tests. It supports excitation with a 405 nm violet laser and fluorescence detection around 550 nm using compatible flow cytometry or microscopy settings. Cell Explorer™ Live Cell Labeling Kit supports live-cell analysis, cell adhesion and chemotaxis studies, and fluorescence-based cell assays.

Citations

View all 4 citations: Citation Explorer

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

Size control and biological properties of monodispersed mesoporous bioactive glass sub-micron spheres

Authors:

Hu, Qing and Li, Yuli and Miao, Guohou and Zhao, Naru and Chen, Xiaofeng

Journal:

Rsc Advances (2014): 22678--22687

Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies

Authors:

Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo

Journal:

Analytical chemistry (2014): 6088--6094

Versatile fabrication of nanoscale sol--gel bioactive glass particles for efficient bone tissue regeneration

Authors:

Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan

Journal:

Journal of Materials Chemistry (2012): 16906--16913

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

G protein-coupled receptor internalization assays in the high-content screening format

Authors:

Haasen D, Schnapp A, Valler MJ, Heilker R.

Journal:

Methods Enzymol (2006): 121

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	525/50 nm filter
Instrument specification(s)	Pacific Orange channel

Fluorescence microscope
Excitation	405 nm
Emission	545/25 filter
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: CytoCalcein™ Violet 550	2 vials
Component B: HHBS (Hanks' buffer with 20 mM Hepes)	1 bottle (100 mL)