Cell Explorer™ Live Cell Tracking Kit *Deep Red Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Platform
Flow cytometer
Excitation | 640 nm laser |
Emission | 660/20 nm filter |
Instrument specification(s) | APC channel |
Fluorescence microscope
Excitation | Cy5 filter |
Emission | Cy5 filter |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples
- Add 10X Track It™ Deep Red working solution (10 µL/well)
- Stain the cells at 37 oC for 15 minutes to 1 hour
- Wash the cells
- Examine the specimen under fluorescence microscope with Cy5 filter or flow cytometer with 660/20 nm filter (APC channel)
Important notes
Thaw all the components to room temperature. Centrifuge the component A briefly before opening.
PREPARATION OF WORKING SOLUTION
Dilute 500X Track It™ Deep Red DMSO stock solution (Component A) into Assay Buffer (Component B) to make a 10 to 25X Track It™ Deep Red working solution. The working solution should be prepared enough for all the wells at 10 µL/well with the appropriate concentration. For example, to get a 10X final concentration of Track It™ Deep Red for one 96-well microplate, dilute 20 µL of 500X Track It™ Deep Red DMSO stock solution into 1 mL of Assay Buffer (Component B) to make 1 mL of 10X Track It™ Deep Red working solution. Note: The final concentration of the Track It™ Deep Red working solution should be empirically determined for different cell types and/or experimental conditions. It is recommended to test at the concentrations at least over a ten fold range. Note: The unused portion of the Track It™ Deep Red stock solution should be stored at -20 oC. Avoid repeated freeze/thaw cycles.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Add 10X Track It™ Deep Red working solution to the cell wells which should be equal to 1/10 of the volume of cell culture medium. For example, for a 96-well plate, add 10 µL/well of 10X Track It™ Deep Red working solution into the cells.
- Incubate the cells in a 37°C, 5% CO2 incubator for 15 minutes to 1 hour.
- Wash cells with Hanks and 20 mM Hepes buffer (HHBS) or an appropriate buffer.
- Fill the cell wells with growth medium.
- Analyze the cells using a fluorescence microscope with Cy5 filter or flow cytometer with 660/20 nm filter (APC channel).
Images
Citations
Authors: Cemma, Marija and Grinstein, Sergio and Brumell, John H
Journal: Autophagy (2016): 1440--1446
Authors: Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo
Journal: Analytical chemistry (2014): 6088--6094
Authors: Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan
Journal: Journal of Materials Chemistry (2012): 16906--16913
References
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Application notes
FAQ
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