Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence*

Cell Explorer™ Live Cell Tracking Kit

Orange Fluorescence

Cell Explorer™ Live Cell Tracking Kit is a fluorescence-based kit for labeling living cells with an intracellularly retained orange fluorescent signal for cell tracking studies.

Esterase-activated labeling: Intracellular esterases convert the weakly fluorescent substrate into a strongly fluorescent product
Cytoplasmic retention: Generates a hydrophilic fluorescent product that is retained in the cell cytoplasm
Orange fluorescence detection: Supports TRITC-compatible microscopy and flow cytometry using a 488 nm or 532 nm laser with a 575/26 nm emission filter
Applications: Suitable for cell tracking, multicolor flow cytometry, cell adhesion and chemotaxis studies

Image of Hela cells stained with Cell Explorer™ Live Cell Labeling Kit *Orange Fluorescence<em>* </em>(Cat#22622)<em> </em>in 96-well Costar black wall/clear bottom. Cells were stained with Track It™ Orange for 15 minutes and image was aquired with fluorescence microscope using TRITC filter.

Protocol

SDS

Catalog	Size	Price	Quantity
22622	200 Tests	Price

Overview

Cell Explorer™ Live Cell Tracking Kit is designed for orange fluorescent labeling of living cells using Track It™ Orange. The cell-permeable, weakly fluorescent substrate enters cells, where intracellular esterases hydrolyze it to produce a strongly fluorescent, hydrophilic product. Retention of this product in the cytoplasm enables fluorescence-based observation of labeled cells.

The kit includes Track It™ Orange supplied as a DMSO stock solution and Assay Buffer, with sufficient reagents for 200 tests. Orange fluorescence can be detected using TRITC-compatible microscopy or flow cytometry with a 488 nm or 532 nm laser and a 575/26 nm emission filter. Cell Explorer™ Live Cell Tracking Kit supports cell tracking, multicolor flow cytometry, cell adhesion and chemotaxis studies.

Citations

View all 7 citations: Citation Explorer

Spheroid Cell Aggregation Enhanced by Enzyme-Free Ultrasound-Detached Cells

Authors:

van Delft, Julien and Imashiro, Chikahiro and Kurashina, Yuta and Hirano, Makoto and Homma, Jun and Mochizuki, Shinsuke and Shimozawa, Hideharu and Takemura, Kenjiro

Journal:

Advanced Biology (2025): e00092

Combining molecular characteristics and therapeutic analysis of PDOs predict clinical responses and guide PDAC personalized treatment

Authors:

Li, Peng and Huang, Minli and Li, Mengyao and Li, Gen and Ma, Yifan and Zhao, Yong and Wang, Xiaowu and Zhang, Yongbin and Shi, Changhong

Journal:

Journal of Experimental \& Clinical Cancer Research (2025): 72

Tricultured cell sheets develop into functional pancreatic islet tissue with a vascular network

Authors:

Sekine, Hidekazu and Homma, Jun and Shimizu, Tatsuya

Single Cell Bioprinting with Ultrashort Laser Pulses

Authors:

Zhang, Jun and Byers, Patrick and Erben, Amelie and Frank, Christine and Schulte-Spechtel, Levin and Heymann, Michael and Docheva, Denitsa and Huber, Heinz P and Sudhop, Stefanie and Clausen-Schaumann, Hauke

Journal:

Advanced Functional Materials (2021): 2100066

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening

Authors:

O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.

Journal:

Arch Toxicol (2006): 580

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	488 nm or 532 nm laser
Emission	575/26 nm filter
Instrument specification(s)	PE channel

Fluorescence microscope
Excitation	540 nm
Emission	570 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Cy3/TRITC filter

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 23, 2026

Component A: Track It™ Orange	1 vial (50 µL - 500X DMSO stock solution)
Component B: Assay Buffer	1 bottle (20 mL)