Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence*

Cell Explorer™ Live Cell Tracking Kit

Orange Fluorescence

Our Cell Explorer™ Live Cell Tracking Kit uses a proprietary orange fluorescent dye that gets enhanced fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The tracking dye has good photostability with robust imaging performance. The kit is particularly suitable for multicolor flow cytometric analysis of cells. It can also be used with a fluorescence microscope equipped with a TRITC filter set. This kit provides an effective tool of labeling cells for flow cytometric and fluorescence microscopic investigations of cellular functions. The effective labeling of cells offers a powerful method for studying cellular events in a spatial and temporal context. This kit is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22622	200 Tests	Price

Citations

View all 7 citations: Citation Explorer

Spheroid Cell Aggregation Enhanced by Enzyme-Free Ultrasound-Detached Cells

Authors:

van Delft, Julien and Imashiro, Chikahiro and Kurashina, Yuta and Hirano, Makoto and Homma, Jun and Mochizuki, Shinsuke and Shimozawa, Hideharu and Takemura, Kenjiro

Journal:

Advanced Biology (2025): e00092

Combining molecular characteristics and therapeutic analysis of PDOs predict clinical responses and guide PDAC personalized treatment

Authors:

Li, Peng and Huang, Minli and Li, Mengyao and Li, Gen and Ma, Yifan and Zhao, Yong and Wang, Xiaowu and Zhang, Yongbin and Shi, Changhong

Journal:

Journal of Experimental \& Clinical Cancer Research (2025): 72

Tricultured cell sheets develop into functional pancreatic islet tissue with a vascular network

Authors:

Sekine, Hidekazu and Homma, Jun and Shimizu, Tatsuya

Single Cell Bioprinting with Ultrashort Laser Pulses

Authors:

Zhang, Jun and Byers, Patrick and Erben, Amelie and Frank, Christine and Schulte-Spechtel, Levin and Heymann, Michael and Docheva, Denitsa and Huber, Heinz P and Sudhop, Stefanie and Clausen-Schaumann, Hauke

Journal:

Advanced Functional Materials (2021): 2100066

Autophagy proteins are not universally required for phagosome maturation

Authors:

Cemma, Marija and Grinstein, Sergio and Brumell, John H

Journal:

Autophagy (2016): 1440--1446

References

View all 26 references: Citation Explorer

Requirements, features, and performance of high content screening platforms

Authors:

Gough AH, Johnston PA.

Journal:

Methods Mol Biol (2007): 41

Past, present, and future of high content screening and the field of cellomics

Authors:

Taylor DL., undefined

Journal:

Methods Mol Biol (2007): 3

Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays

Authors:

Giuliano KA., undefined

Journal:

Methods Mol Biol (2007): 189

A pharmaceutical company user's perspective on the potential of high content screening in drug discovery

Authors:

Hoffman AF, Garippa RJ.

Journal:

Methods Mol Biol (2007): 19

High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening

Authors:

O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.

Journal:

Arch Toxicol (2006): 580

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm or 532 nm laser
Emission	575/26 nm filter
Instrument specification(s)	PE channel

Fluorescence microscope
Excitation	540 nm
Emission	570 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Cy3/TRITC filter

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 25, 2025

Component A: Track It™ Orange	1 vial (50 µL - 500X DMSO stock solution)
Component B: Assay Buffer	1 bottle (20 mL)