Cell Explorer™ Live Cell Tracking Kit *Red Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Platform
Flow cytometer
Excitation | 488 nm or 561 nm laser |
Emission | 610/20 nm filter |
Instrument specification(s) | PE-Texas Red channel |
Fluorescence microscope
Excitation | 570 nm |
Emission | 600 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Texas Red filter set |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples
- Add 10X Track It™ Red working solution (10 µL/well)
- Incubate the cells at 37°C for 15 to 60 minutes
- Wash the cells
- Examine the specimen under fluorescence microscope with Texas Red filter or flow cytometer with 610/20 nm filter (PE-Texas Red channel)
Important notes
Thaw all the components at room temperature. Centrifuge the Component A brieftly before opening.
PREPARATION OF WORKING SOLUTION
Dilute 500X Track It™ Red DMSO stock solution (Component A) into Assay Buffer (Component B) to make a 10 to 25X Track It™ Red working solution. The working solution should be prepared enough for all the wells at 10 μL/well with the appropriate concentration. For example, to get a 10X final concentration of Track It™ Red for one 96-well microplate, dilute 20 μL of 500X Track It™ Red DMSO stock solution into 1 mL of Assay Buffer (Component B) to make 1 mL of 10X Track It™ Red working solution. Note: The final concentration of the Track It™ Red working solution should be empirically determined for different cell types and/or experimental conditions. It is recommended to test at the concentrations that are at least over a ten fold range.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Add 10X Track It™ Red working solution to the cell wells which should be equal to 1/10 of the volume of cell culture medium. For example, for a 96-well plate, add 10 µL/well of 10X Track It™ Blue working solution into the cells.
- Incubate the cells in a 37°C, 5% CO2 incubator for 15 to 60 minutes.
- Wash cells with Hanks and 20 mM Hepes buffer (HHBS) or an appropriate buffer.
- Fill the cell wells with growth medium.
- Analyze the cells using a fluorescence microscope with Texas Red filter or flow cytometer with 610/20 nm filter (PE-Texas Red channel).
Images
Citations
Authors: Zha, Linjun and Matsu-ura, Toru and Sluka, James P and Murakawa, Tomohiro and Tsuta, Koji
Journal: iScience (2024): 109742
Authors: Cemma, Marija and Grinstein, Sergio and Brumell, John H
Journal: Autophagy (2016): 1440--1446
Authors: Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo
Journal: Analytical chemistry (2014): 6088--6094
Authors: Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan
Journal: Journal of Materials Chemistry (2012): 16906--16913
References
Authors: Gough AH, Johnston PA.
Journal: Methods Mol Biol (2007): 41
Authors: Hoffman AF, Garippa RJ.
Journal: Methods Mol Biol (2007): 19
Authors: Giuliano KA., undefined
Journal: Methods Mol Biol (2007): 189
Authors: Taylor DL., undefined
Journal: Methods Mol Biol (2007): 3
Authors: Martinez ED, Dull AB, Beutler JA, Hager GL.
Journal: Methods Enzymol (2006): 21
Authors: Bowen WP, Wylie PG.
Journal: Assay Drug Dev Technol (2006): 209
Authors: Hudson CC, Oakley RH, Sjaastad MD, Loomis CR.
Journal: Methods Enzymol (2006): 63
Authors: Werner T, Liebisch G, Gr and l M, Schmitz G.
Journal: Cytometry A (2006): 200
Authors: Wolff M, Haasen D, Merk S, Kroner M, Maier U, Bordel S, Wiedenmann J, Nienhaus GU, Valler M, Heilker R.
Journal: Comb Chem High Throughput Screen (2006): 339
Authors: O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.
Journal: Arch Toxicol (2006): 580
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Buccutite™ Bioconjugation Technology
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins
FAQ
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
How do I stain T cells with CytoTell™ Blue?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you have fixable live cell staining assay kits?