Name: Cell Meter™ Annexin V Apoptosis Assay Kit
Brand: AAT Bioquest
SKU: 22824
Price: 237 USD
Availability: InStock

Cell Meter™ Annexin V Binding Apoptosis Assay Kit

Green Fluorescence Optimized for Flow Cytometry

Cell Meter™ Annexin V Binding Apoptosis Assay Kit is a fluorimetric assay kit for detecting apoptosis through phosphatidylserine (PS) externalization.

Detection Principle: Uses Annexin V-iFluor® 488 fluorescence to detect apoptotic cells.
Sensitivity: Detects early apoptosis before morphological changes occur.
Binding Specificity: Annexin V selectively binds exposed phosphatidylserine.
Instrument Compatibility: Compatible with 488 nm laser flow cytometry (FITC channel).
Applications: Monitors early- and intermediate-stage apoptosis.

The detection of binding activity of Annexin V-iFluor® 488 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with Annexin V-iFluor® 488 for 30 minutes. The fluorescence intensity of Annexin V-iFluor® 488 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using the FL1 channel.

Protocol

SDS

Catalog	Size	Price	Quantity
22824	100 Tests	Price

Overview

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses a fluorescent Annexin V that specifically binds PS. Annexin V conjugates have been demonstrated to selectively bind PS. This particular assay kit is optimized to monitor cell apoptosis using a flow cytometer with the FITC channel (green fluorescence).

Citations

View all 13 citations: Citation Explorer

Design, Synthesis, and In Vivo Photodynamic Efficacy of a Mitochondria-Targeted Triphenylamine--Coumarin AIE Photosensitizer

Authors:

Wang, Zening and He, Xianrui and Min, Shuang and Wang, Douxi and Du, Linghong and Zhang, Lu and Gao, Tao and Wang, Jianglin

Journal:

Dyes and Pigments (2026): 114227

Integration of single-cell and bulk transcriptomics reveals the association of manganese metabolism-related genes with prognosis and immune infiltration in lung adenocarcinoma

Authors:

Li, Hongliang and Wei, Shuzhen and Li, Wei and Sun, Junxia and Wang, Yi

Journal:

Clinical \& Translational Immunology (2026): e70085

CCT6A knockdown suppresses osteosarcoma cell growth and Akt pathway activation in vitro

Authors:

Zeng, Weiquan and Wu, Meizhu and Cheng, Ying and Liu, Liya and Han, Yuying and Xie, Qiurong and Li, Jiapeng and Wei, Lihui and Fang, Yi and Chen, Youqin and others

Journal:

Plos one (2022): e0279851

Phosphatidylserine exposure modulates adhesion GPCR BAI1 (ADGRB1) signaling activity

Authors:

Lala, Trisha and Doan, Juleva K and Takatsu, Hiroyuki and Hartzell, H Criss and Shin, Hye-Won and Hall, Randy A

Journal:

Journal of Biological Chemistry (2022): 102685

Atorvastatin attenuates pulmonary fibrosis in mice and human lung fibroblasts, by the regulation of myofibroblast differentiation and apoptosis

Authors:

Yildirim, Merve and Kayalar, Ozgecan and Atahan, Ersan and Oztay, Fusun

Journal:

Journal of Biochemical and Molecular Toxicology (2022): e23074

References

View all 32 references: Citation Explorer

Gold fluorescent annexin A5 as a novel apoptosis detection tool

Authors:

Kurschus FC, Pal PP, Baumler P, Jenne DE, Wiltschi B, Budisa N.

Journal:

Cytometry A (2009): 626

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection

Authors:

Palma PF, Baggio GL, Spada C, Silva RD, Ferreira SI, Treitinger A.

Journal:

Braz J Infect Dis (2008): 108

Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Authors:

Chen S, Cheng AC, Wang MS, Peng X.

Journal:

World J Gastroenterol (2008): 2174

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells

Authors:

Hu T, Shi J, Jiao X, Zhou J, Yin X.

Journal:

Braz J Med Biol Res (2008): 750

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

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Fax
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Page updated on November 13, 2026

Component A: Annexin V-iFluor® 488 (100X stock solution)	1 vial (200 µL/vial)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: 100X Propidium Iodide	1 vial (100 µL)