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Cell Meter™ Annexin V Binding Apoptosis Assay Kit
Blue Fluorescence Excited at 405 nm
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses a fluorescent Annexin V that specifically binds PS. Annexin V conjugates have been demonstrated to selectively bind PS. This particular assay kit is optimized to monitor cell apoptosis using a flow cytometer with the Pacific Blue filter set.
The detection of binding activity of Annexin V-mFluor™ Violet 450 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for 5 hours, and then dye loaded with Annexin V-mFluor™ Violet 450 for 30 minutes. The fluorescence intensity of Annexin V-mFluor™ Violet 450 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using 405 nm laser at Ex/Em = 405/450 nm.
The detection of binding activity of Annexin V-mFluor™ Violet 450 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for 5 hours, and then dye loaded with Annexin V-mFluor™ Violet 450 for 30 minutes. The fluorescence intensity of Annexin V-mFluor™ Violet 450 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using 405 nm laser at Ex/Em = 405/450 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22828100 Tests
Price
 
Spectral properties
Absorbance (nm)406
Correction factor (260 nm)0.338
Correction factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)
35000
1
Excitation (nm)406
Emission (nm)445
Quantum yield
0.81
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission450/40 nm filter
Instrument specification(s)Pacific Blue channel
Fluorescence microscope
ExcitationDAPI filter set
EmissionDAPI filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024