Cell Meter™ Annexin V Apoptosis Assay Kit

Cell Meter™ Annexin V Binding Apoptosis Assay Kit

Green Fluorescence Excited at 405 nm

Cell Meter™ Annexin V Binding Apoptosis Assay Kit is a fluorimetric assay kit for detecting apoptosis through phosphatidylserine (PS) externalization.

Detection Principle: Uses Annexin V-mFluor™ Violet 500 fluorescence to detect apoptotic cells.
Sensitivity: Detects early apoptosis before morphological changes occur.
Binding Specificity: Annexin V selectively binds exposed phosphatidylserine.
Instrument Compatibility: Compatible with 405 nm laser flow cytometry (AmCyan channel).
Applications: Monitors early- and intermediate-stage apoptosis.

The detection of binding activity of Annexin V-mFluor™ Violet 500 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for 5 hours, and then dye loaded with Annexin V-mFluor™ Violet 500 for 30 minutes. The fluorescence intensity of Annexin V-mFluor™ Violet 500 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using 405 nm laser at Ex/Em = 405/500 nm.

Protocol

SDS

Catalog	Size	Price	Quantity
22829	100 Tests	Price

Overview

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses a fluorescent Annexin V that specifically binds PS. Annexin V conjugates have been demonstrated to selectively bind PS. This particular assay kit is optimized to monitor cell apoptosis using a flow cytometer with the 405 laser with 525/40 emission filter set.

Citations

View all 6 citations: Citation Explorer

CCT6A knockdown suppresses osteosarcoma cell growth and Akt pathway activation in vitro

Authors:

Zeng, Weiquan and Wu, Meizhu and Cheng, Ying and Liu, Liya and Han, Yuying and Xie, Qiurong and Li, Jiapeng and Wei, Lihui and Fang, Yi and Chen, Youqin and others

Journal:

Plos one (2022): e0279851

In situ polymerizable hydrogel incorporated with specific pathogen-free porcine platelet-rich plasma for the reconstruction of the corneal endothelium

Authors:

Lin, Yung-Kai and Sharma, Ruchi and Ma, Hsu and Chen, Wen-Shyan and Yao, Chao-Ling

Journal:

Journal of the Taiwan Institute of Chemical Engineers (2017)

Novel regulations of MEF2-A, MEF2-D, and CACNA1S in the functional incompetence of adipose-derived mesenchymal stem cells by induced indoxyl sulfate in chronic kidney disease

Authors:

Do, Duyen Thi and Phan, Nam Nhut and Wang, Chih-Yang and Sun, Zhengda and Lin, Yen-Chang

Journal:

Cytotechnology (2016): 2589--2604

Shear stress-induced alteration of epithelial organization in human renal tubular cells

Authors:

Maggiorani, Damien and Dissard, Romain and Belloy, Marcy and Saulnier-Blache, Jean-S{\'e}bastien and Casemayou, Audrey and Ducasse, Laure and Gr{\`e}s, Sandra and Belli{\`e}re, Julie and Caubet, C{\'e}cile and Bascands, Jean-Loup and others

Journal:

PLoS One (2015): e0131416

Shear stress-induced alteration of epithelial organization in human renal tubular cells

Authors:

Maggiorani, Damien and Dissard, Romain and Belloy, Marcy and Saulnier-Blache, Jean-Sébastien and Casemayou, Audrey and Ducasse, Laure and Grès, S and ra and Bellière, Julie and Caubet, Cécile and Basc and s, Jean-Loup and others

Journal:

PloS one (2015): e0131416

References

View all 32 references: Citation Explorer

Gold fluorescent annexin A5 as a novel apoptosis detection tool

Authors:

Kurschus FC, Pal PP, Baumler P, Jenne DE, Wiltschi B, Budisa N.

Journal:

Cytometry A (2009): 626

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Evaluation of annexin V and Calcein-AM as markers of mononuclear cell apoptosis during human immunodeficiency virus infection

Authors:

Palma PF, Baggio GL, Spada C, Silva RD, Ferreira SI, Treitinger A.

Journal:

Braz J Infect Dis (2008): 108

Measurement of annexin V uptake and lactadherin labeling for the quantification of apoptosis in adherent Tca8113 and ACC-2 cells

Authors:

Hu T, Shi J, Jiao X, Zhou J, Yin X.

Journal:

Braz J Med Biol Res (2008): 750

Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

Authors:

Chen S, Cheng AC, Wang MS, Peng X.

Journal:

World J Gastroenterol (2008): 2174

Spectral properties

Absorbance (nm)	412
Correction factor (260 nm)	0.769
Correction factor (280 nm)	0.365
Extinction coefficient (cm ^-1 M ^-1)	25000 ¹
Excitation (nm)	410
Emission (nm)	501
Quantum yield	0.81 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	525/40 nm filter
Instrument specification(s)	AmCyan channel

Fluorescence microscope
Excitation	Violet filter set
Emission	Violet filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 2, 2026

Component A: Annexin V-mFluor™ Violet 500 (100X stock solution)	1 vial (200 µL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: 100X Propidium Iodide	1 vial (100 µL)