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Cell Meter™ Annexin V Binding Apoptosis Assay Kit
Orange Fluorescence Excited at 405 nm
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses a fluorescent Annexin V that specifically binds PS. Annexin V conjugates have been demonstrated to selectively bind PS. This particular assay kit is optimized to monitor cell apoptosis using a flow cytometer with the Pacific Orange filter set.
The detection of binding activity of Annexin V-mFluor™ Violet 550 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for 5 hours, and then dye loaded with Annexin V-mFluor™ Violet 550 for 30 minutes. The fluorescence intensity of Annexin V-mFluor™ Violet 550 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using 405 nm laser at Ex/Em = 405/550 nm.
The detection of binding activity of Annexin V-mFluor™ Violet 550 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for 5 hours, and then dye loaded with Annexin V-mFluor™ Violet 550 for 30 minutes. The fluorescence intensity of Annexin V-mFluor™ Violet 550 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using 405 nm laser at Ex/Em = 405/550 nm.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22830100 Tests
Price
 
Spectral properties
Absorbance (nm)527
Correction factor (260 nm)0.474
Correction factor (280 nm)0.306
Extinction coefficient (cm -1 M -1)
90000
1
Excitation (nm)420
Emission (nm)550
Quantum yield
0.31
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission525/50 nm filter
Instrument specification(s)Pacific Orange channel
Fluorescence microscope
ExcitationViolet filter set
EmissionViolet filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024