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Cell Meter™ Annexin V Binding Apoptosis Assay Kit
Orange Fluorescence Optimized for Flow Cytometry
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses a fluorescent Annexin V that specifically binds PS. Annexin V conjugates have been demonstrated to selectively bind PS. This particular assay kit is optimized to monitor cell apoptosis using a flow cytometer with 575/26 nm emission filter set (PE channel).
Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with Cell Meter Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence*. (Left): Untreated control cells. (Right): Cells treated with 1 μM staurosporine for 5 hours.
Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with Cell Meter Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence*. (Left): Untreated control cells. (Right): Cells treated with 1 μM staurosporine for 5 hours.
CatalogSize
Price
Quantity
22825100 Tests
Price
 
Spectral properties

Correction factor (260 nm)0.23
Correction factor (280 nm)0.14
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)557
Emission (nm)570
Quantum yield
0.64
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation488 nm or 532 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
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Page updated on October 8, 2024