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Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit
Optimized for Flow Cytometry
Annexin V may be conjugated to fluorochromes including APC. This format retains its high affinity for phosphatidylserine (PS) and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, APC Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation. APC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with APC Annexin V is typically used in conjunction with a vital dye such as propidium iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells.
The detection of binding activity of APC-Annexin V to phosphatidylserine in Jurkat cells with Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for ~4 hours, and then dye loaded with APC-Annexin V for 30 minutes. The fluorescence intensity of APC-Annexin V was measured with a FACSCalibur (Becton Dickinson) flow cytometer using the FL4 channel.
The detection of binding activity of APC-Annexin V to phosphatidylserine in Jurkat cells with Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for ~4 hours, and then dye loaded with APC-Annexin V for 30 minutes. The fluorescence intensity of APC-Annexin V was measured with a FACSCalibur (Becton Dickinson) flow cytometer using the FL4 channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22837100 Tests
Price
 
Spectral properties
Correction factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
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Page updated on October 8, 2024