Cell Meter™ Annexin V Apoptosis Assay Kit

Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit

Optimized for Flow Cytometry

Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit is a fluorimetric assay kit for detecting apoptosis through phosphatidylserine (PS) externalization.

Detection Principle: Uses APC-Annexin V fluorescence to detect apoptotic cells.
Sensitivity: Detects early apoptosis before nuclear DNA fragmentation.
Binding Specificity: APC-Annexin V selectively binds exposed phosphatidylserine.
Instrument Compatibility: Compatible with 640 nm laser flow cytometry (APC channel).
Applications: Differentiates early apoptotic and nonviable cells using optional PI staining.

The detection of binding activity of APC-Annexin V to phosphatidylserine in Jurkat cells with Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for ~4 hours, and then dye loaded with APC-Annexin V for 30 minutes. The fluorescence intensity of APC-Annexin V was measured with a FACSCalibur (Becton Dickinson) flow cytometer using the FL4 channel.

Protocol

SDS

Catalog	Size	Price	Quantity
22837	100 Tests	Price

Overview

Annexin V may be conjugated to fluorochromes including APC. This format retains its high affinity for phosphatidylserine (PS) and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis. Since externalization of PS occurs in the earlier stages of apoptosis, APC Annexin V staining can identify apoptosis at an earlier stage than assays based on nuclear changes such as DNA fragmentation. APC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes. Therefore, staining with APC Annexin V is typically used in conjunction with a vital dye such as propidium iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells.

Citations

View all 11 citations: Citation Explorer

Neutrophil serine proteases are internalized by glioblastoma cells and modulate cell mechanics, apoptosis, and migration

Authors:

Shagirova, Alua and Allayarova, Maiya and Makhanova, Aiya and Kalendar, Ruslan and Westhoff, Mike-Andrew and Filchakova, Olena and Bekturgan, Amanbek and Toleubekova, Malika and Ormantayeva, Anar and Pham, Tri Thanh and others

BRAF D594A mutation defines a unique biological and immuno-modulatory subgroup associated with functional CD8+ T cell infiltration in colorectal cancer

Authors:

Li, Wenjing and Zhao, Chenyi and Li, Wenhui and Gong, Yang and Ma, Kaili and Lu, Yujie and Liu, Xiaowei and Zhang, Lianjun and Guo, Feng

Upregulation of LPGAT1 Enhances Lung Adenocarcinoma Proliferation

Authors:

Gong, Huiyuan and Ma, Chao and Li, Xiaojun and Zhang, Xueying and Zhang, Linxiang and Chen, Pengfei and Wang, Wei and Hu, Yannan and Huang, Ting and Wu, Nan and others

Journal:

Frontiers in Bioscience-Landmark (2023): 89

Th22/IL-22 mediates the progression of HBV-related hepatocellular carcinoma via STAT3

Authors:

Zhang, Jia and Liu, Zhou and Liu, Lingpeng and Huang, Mingwen and Huang, Yong

Journal:

Cytotechnology (2022): 203--216

Effects of Y-27632 on the osteogenic and adipogenic potential of human dental pulp stem cells in vitro

Authors:

Xie, Zhiwei and Xu, Qiuping and Sun, Lu and Li, Ruijing and Shi, Jizhou and Yang, Qian and Zong, Min and Qin, Jianyong

Journal:

Human \& Experimental Toxicology (2022): 09603271221089003

References

View all 135 references: Citation Explorer

Detection of apoptosis based on the interaction between annexin V and phosphatidylserine

Authors:

Liu T, Zhu W, Yang X, Chen L, Yang R, Hua Z, Li G.

Journal:

Anal Chem (2009): 2410

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Trivalent methylated arsenical-induced phosphatidylserine exposure and apoptosis in platelets may lead to increased thrombus formation

Authors:

Bae ON, Lim KM, Noh JY, Chung SM, Kim SH, Chung JH.

Journal:

Toxicol Appl Pharmacol (2009): 144

Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry

Authors:

Wlodkowic D, Skommer J, Faley S, Darzynkiewicz Z, Cooper JM.

Journal:

Exp Cell Res (2009): 1706

Eurycomanone induce apoptosis in HepG2 cells via up-regulation of p53

Authors:

Zakaria Y, Rahmat A, Pihie AH, Abdullah NR, Houghton PJ.

Journal:

Cancer Cell Int (2009): 16

Spectral properties

Correction factor (280 nm)	0.195
Extinction coefficient (cm ^-1 M ^-1)	730000
Excitation (nm)	651
Emission (nm)	660

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	640 nm laser
Emission	660/20 nm filter
Instrument specification(s)	APC channel

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Page updated on October 2, 2026

Component A: APC-Annexin V conjugate	1 Vial
Component B: Assay Buffer (4 °C)	1 Bottle (50 mL)
Component C: 100X Propidium Iodide	1 Vial (100 µL)