Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence Colors*

Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I

Triple Fluorescence Colors

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to monitor cell apoptotic, necrotic and healthy cells. Apoptosis is described as an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed. The PS sensor used in this kit has green fluorescence upon binding to membrane PS. Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable 7-AAD (Ex/Em = 546/647 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis. In addition, this kit also provides a live cell cytoplasm labeling dye CytoCalcein™ Violet 450 (Ex/Em = 405/450 nm) for labeling living cell cytoplasm. This kit is optimized to detect cell apoptosis (green), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer and fluorescence microscope.

The fluorescence images showing cells that are live (blue, stained by CytoCalcein™ Violet 450), apoptotic (green, stained by Apopxin™ Green), and necrotic (red, indicated by 7-AAD staining) in Jurkat cells induced by 1µM staurosporine for 3 hours. The fluorescence images of the cells were taken with Olympus fluorescence microscope through the Violet, FITC and Texas Red channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. Left: Non-induced control cells; Right: Triple staining of staurosporine-induced cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22840	100 Tests	Price

Citations

View all 10 citations: Citation Explorer

Apoptotic Effect of Combinations of T-2, HT-2, and Diacetoxyscirpenol on Human Jurkat T Cells

Authors:

Wattanasuntorn, Phattarawadee and Poapolathep, Saranya and Phuektes, Patchara and Alassane-Kpembi, Imourana and Fink-Gremmels, Johanna and Oswald, Isabelle P and Poapolathep, Amnart

Journal:

Toxins (2025): 203

Oxygen/Nitric Oxide Dual-Releasing Nanozyme for Augmenting TMZ-Mediated Apoptosis and Necrosis

Authors:

Ma, Jun and Qiu, Jingjing and Wright, Gus A and Wang, Shiren

Journal:

Molecular Pharmaceutics (2024)

Effect of oligosaccharyl transglycosylation on the cytotoxicity of cardiac glycoside proscillaridin A and its ability to inhibit hepatitis B virus infection

Authors:

Wakamatsu, Akira and Fujisawa, Yu and Ohashi, Hiroyuki and Kinoshita, Takashi and Dozaki, Masahito and Suda, Minoru and Honda, Tomoyuki and Ohashi, Takao and Kajiura, Hiroyuki and Ueda, Keiji and others,

Co-expression of SLC20A1 and ALDH1A3 is associated with poor prognosis, and SLC20A1 is required for the survival of ALDH1-positive pancreatic cancer stem cells

Authors:

Matsuoka, Izumi and Kasai, Takahiro and Onaga, Chotaro and Ozaki, Ayaka and Motomura, Hitomi and Maemura, Yuki and Tada, Yuna and Mori, Haruka and Hara, Yasushi and Xiong, Yuyun and others,

Journal:

Oncology Letters (2024): 1--16

Mesenchymal Stem Cells Preconditioned with Hypoxia and Dexamethasone Promote Osteoblast Differentiation Under Stress Conditions

Authors:

Shimasaki, Miyako and Ichiseki, Toru and Ueda, Shusuke and Hirata, Hiroaki and Kawahara, Norio and Ueda, Yoshimichi

Journal:

International Journal of Medical Sciences (2024): 1511--1517

References

View all 71 references: Citation Explorer

Susceptibility of the C2 canine mastocytoma cell line to the effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)

Authors:

Elders RC, Baines SJ, Catchpole B.

Journal:

Vet Immunol Immunopathol (2009): 11

PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes

Authors:

Foldbjerg R, Olesen P, Hougaard M, Dang DA, Hoffmann HJ, Autrup H.

Journal:

Toxicol Lett (2009): 156

Concurrent induction of necrosis, apoptosis, and autophagy in ischemic preconditioned human livers formerly treated by chemotherapy

Authors:

Domart MC, Esposti DD, Sebagh M, Olaya N, Harper F, Pierron G, Franc B, Tanabe KK, Debuire B, Azoulay D, Brenner C, Lemoine A.

Journal:

J Hepatol (2009): 881

Rapid simultaneous determination of apoptosis, necrosis, and viability in sulfur mustard exposed HaCaT cell cultures

Authors:

Heinrich A, Balszuweit F, Thiermann H, Kehe K.

Journal:

Toxicol Lett (2009): 260

RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects

Authors:

Kreeger PK, M and hana R, Alford SK, Haigis KM, Lauffenburger DA.

Journal:

Cancer Res (2009): 8191

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	405 nm, 488 nm laser
Emission	450/40 nm, 530/30 nm, 670/14 nm filter
Instrument specification(s)	Pacific Blue, FITC, PE-Cy5 channel

Fluorescence microscope
Excitation	DAPI, FITC, Texas Red filter
Emission	DAPI, FITC, Texas Red filter
Recommended plate	Black wall/clear bottom

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Fax
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 6, 2025

Component A: 100X Apopxin™ Green	1 vial (200 µL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: 200X 7-AAD	1 vial (100 µL)
Component D: CytoCalcein™ Violet 450	1 vial (lyophilized powder)