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Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II
Triple Fluorescence Colors
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to simultaneously monitor apoptotic, necrotic, and healthy cells. Apoptosis is described as an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed. The PS sensor used in this kit has red fluorescence upon binding to membrane PS. Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with the uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable DNA Nuclear Green™ DCS1 (Ex/Em = 490/525 nm) to label the nucleus, represents a straightforward approach to demonstrate late-stage apoptosis and necrosis. In addition, this kit also provides a live cell cytoplasm labeling dye, CytoCalcein™ Violet 450 (Ex/Em = 405/450 nm), for labeling living cell cytoplasm. This kit is optimized to simultaneously detect cell apoptosis (red and/or green), necrosis (green), and healthy cells (blue) with a flow cytometer or fluorescence microscope.
The detection of binding activity of Apopxin™ Deep Red to phosphatidylserine in Jurkat cells using Cell Meter™ Apoptotic and Necrotic Mulptiplexing Detection Kit II. The fluorescence images showing cells that are live (blue, stained by CytoCalcein™ Violet 450), apoptotic (red, stained by Apopxin™ Deep Red), and necrotic (green, indicated by Nuclear Green™ DCS1 staining) in Jurkat cells induced by 1 μM staurosporine for 3 hours. The fluorescence images of the cells were taken with Olympus fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Triple staining of staurosporine-induced cells.
The detection of binding activity of Apopxin™ Deep Red to phosphatidylserine in Jurkat cells using Cell Meter™ Apoptotic and Necrotic Mulptiplexing Detection Kit II. The fluorescence images showing cells that are live (blue, stained by CytoCalcein™ Violet 450), apoptotic (red, stained by Apopxin™ Deep Red), and necrotic (green, indicated by Nuclear Green™ DCS1 staining) in Jurkat cells induced by 1 μM staurosporine for 3 hours. The fluorescence images of the cells were taken with Olympus fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Triple staining of staurosporine-induced cells.
CatalogSize
Price
Quantity
22843100 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation405 nm, 488 nm, 633 nm laser
Emission450/40 nm, 530/30 nm, 660/20 nm filter
Instrument specification(s)Pacific Blue, FITC, APC channel

Fluorescence microscope
ExcitationDAPI, FITC, Cy5 filter sets
EmissionDAPI, FITC, Cy5 filter sets
Recommended plateBlack wall/clear bottom
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Page updated on September 25, 2025