Name: Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II *Triple Fluorescence Colors*
Brand: AAT Bioquest
SKU: 22843
Price: 348 USD
Availability: InStock

Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II

Triple Fluorescence Colors

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to simultaneously monitor apoptotic, necrotic, and healthy cells. Apoptosis is described as an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed. The PS sensor used in this kit has red fluorescence upon binding to membrane PS. Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with the uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable DNA Nuclear Green™ DCS1 (Ex/Em = 490/525 nm) to label the nucleus, represents a straightforward approach to demonstrate late-stage apoptosis and necrosis. In addition, this kit also provides a live cell cytoplasm labeling dye, CytoCalcein™ Violet 450 (Ex/Em = 405/450 nm), for labeling living cell cytoplasm. This kit is optimized to simultaneously detect cell apoptosis (red and/or green), necrosis (green), and healthy cells (blue) with a flow cytometer or fluorescence microscope.

The detection of binding activity of Apopxin™ Deep Red to phosphatidylserine in Jurkat cells using Cell Meter™ Apoptotic and Necrotic Mulptiplexing Detection Kit II. The fluorescence images showing cells that are live (blue, stained by CytoCalcein™ Violet 450), apoptotic (red, stained by Apopxin™ Deep Red), and necrotic (green, indicated by Nuclear Green™ DCS1 staining) in Jurkat cells induced by 1 μM staurosporine for 3 hours. The fluorescence images of the cells were taken with Olympus fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Triple staining of staurosporine-induced cells.

Protocol

SDS

Catalog	Size	Price	Quantity
22843	100 Tests	Price

Citations

View all 6 citations: Citation Explorer

Transcriptional coregulator Ess2 controls survival of post-thymic CD4+ T cells through the Myc and IL-7 signaling pathways

Authors:

Takada, Ichiro and Hidano, Shinya and Takahashi, Sayuri and Yanaka, Kaori and Ogawa, Hidesato and Tsuchiya, Megumi and Yokoyama, Atsushi and Sato, Shingo and Ochi, Hiroki and Nakagawa, Tohru and others,

Journal:

Journal of Biological Chemistry (2022)

Dasatinib can Impair Left Ventricular Mechanical Function But May Lack Proarrhythmic Effect: A Proposal of Non-clinical Guidance for Predicting Clinical Cardiovascular Adverse Events of Tyrosine Kinase Inhibitors

Authors:

Izumi-Nakaseko, Hiroko and Fujiyoshi, Masachika and Hagiwara-Nagasawa, Mihoko and Goto, Ai and Chiba, Koki and Kambayashi, Ryuichi and Naito, Atsuhiko T and Ando, Kentaro and K, undefined and a, Yasunari and Ishii, Itsuko and others, undefined

Journal:

Cardiovascular Toxicology (2019): 1--13

Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

Authors:

Nanashima, Naoki and Horie, Kayo and Chiba, Mitsuru and Nakano, Manabu and Maeda, Hayato and Nakamura, Toshiya

Journal:

Molecular Medicine Reports (2017): 6134--6141

Clusterin signals via ApoER2/VLDLR and induces meiosis of male germ cells

Authors:

Riaz, Muhammad Assad and Stammler, Angelika and Borgers, Mareike and Konrad, Lutz

Journal:

American Journal of Translational Research (2017): 1266

Detecting Apoptosis, Autophagy, and Necrosis

Authors:

Coleman, Jack and Liu, Rui and Wang, Kathy and Kumar, Arun

Journal:

Apoptosis Methods in Toxicology (2016): 77--92

References

View all 71 references: Citation Explorer

PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes

Authors:

Foldbjerg R, Olesen P, Hougaard M, Dang DA, Hoffmann HJ, Autrup H.

Journal:

Toxicol Lett (2009): 156

Concurrent induction of necrosis, apoptosis, and autophagy in ischemic preconditioned human livers formerly treated by chemotherapy

Authors:

Domart MC, Esposti DD, Sebagh M, Olaya N, Harper F, Pierron G, Franc B, Tanabe KK, Debuire B, Azoulay D, Brenner C, Lemoine A.

Journal:

J Hepatol (2009): 881

Rapid simultaneous determination of apoptosis, necrosis, and viability in sulfur mustard exposed HaCaT cell cultures

Authors:

Heinrich A, Balszuweit F, Thiermann H, Kehe K.

Journal:

Toxicol Lett (2009): 260

RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects

Authors:

Kreeger PK, M and hana R, Alford SK, Haigis KM, Lauffenburger DA.

Journal:

Cancer Res (2009): 8191

Susceptibility of the C2 canine mastocytoma cell line to the effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)

Authors:

Elders RC, Baines SJ, Catchpole B.

Journal:

Vet Immunol Immunopathol (2009): 11

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	405 nm, 488 nm, 633 nm laser
Emission	450/40 nm, 530/30 nm, 660/20 nm filter
Instrument specification(s)	Pacific Blue, FITC, APC channel

Fluorescence microscope
Excitation	DAPI, FITC, Cy5 filter sets
Emission	DAPI, FITC, Cy5 filter sets
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Enzyme Probes & Assay Kits

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 5, 2025

Component A: 100X Apopxin™ Deep Red	1 vial (200 µL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: 200X Nuclear Green™ DCS1	1 vial (100 µL)
Component D: CytoCalcein™ Violet 450	1 vial (lyophilized powder)