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Cell Meter™ Beta-Arrestin Translocation GPCR Signaling Kit
Virtually all G protein coupled receptors (GPCRs) rapidly undergo desensitization by a common pathway upon activation by ligand binding. The binding of beta-arrestin, a cytoplasmic protein, to an activated receptor deactivates the GPCR signaling and initiates the translocation of the receptor into the cell where the ligand is removed, and the receptor is recycled back to the cell membrane. By attaching a fluorescent label, such as GFP, to beta-arrestin, the location of the receptor arrestin complex can be monitored. Since desensitization only occurs with an activated receptor, monitoring beta-arrestin translocation and subsequent receptor recycling provides a reliable method to detect the activation of a GPCR target. Cell Meter™ Beta-Arrestin translocation GPCR Signaling Kit provides a powerful functional assay to screen activities of target compounds against known or orphan GPCR targets via fluorescence imaging. The activation of the targeted GPCR induces the translocation of the fluorescence to the cell membrane and/or to endocytic vesicles.
Translocation of beta-arrestin in HeLa cells. HeLa cells were transiently transfected with beta-arrestin-GFP and vasopressin receptor 2 (V2R). HeLa cells were cultured in a 6-well plate and grown to ~60% confluence.  Equal amounts of beta-arrestin-GFP (1.5 µg) and V2R plasmids (1.5 µg) were transfected with 9 µL of Transfectamine™ 5000. Cells were transferred to a 96-well plate ~ 30 hours after transfection. Vasopressin (1 µM) was added to the cells ~ 48 hours after transfection to induce beta-arrestin-GFP translocation. Images were taken before and 2 hours after the vasopressin treatment under a fluorescent microscope using the FITC channel.
Translocation of beta-arrestin in HeLa cells. HeLa cells were transiently transfected with beta-arrestin-GFP and vasopressin receptor 2 (V2R). HeLa cells were cultured in a 6-well plate and grown to ~60% confluence.  Equal amounts of beta-arrestin-GFP (1.5 µg) and V2R plasmids (1.5 µg) were transfected with 9 µL of Transfectamine™ 5000. Cells were transferred to a 96-well plate ~ 30 hours after transfection. Vasopressin (1 µM) was added to the cells ~ 48 hours after transfection to induce beta-arrestin-GFP translocation. Images were taken before and 2 hours after the vasopressin treatment under a fluorescent microscope using the FITC channel.
protocol
Protocol
COA
CatalogSize
Price
Quantity
36390100 Tests
Price
36391200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 4, 2025