Cell Meter™ BX590 fixable viability dye

Cell Meter™ BX590 Fixable Viability Dye is a fluorimetric reagent for distinguishing live and dead mammalian cells based on membrane integrity.

Detection Principle: Uses BX590 fluorescence to distinguish live and dead cells.
Signal Advantage: Provides robust, stable fluorescence retained after fixation.
Reaction Chemistry: Irreversibly binds amine- and thiol-containing cellular components.
Instrument Compatibility: Compatible with 488 nm laser flow cytometry (PE channel).
Applications: Identifies and excludes dead cells during flow cytometric analysis.

Detection of Jurkat cell viability by Cell Meter™ fixable viability dye. Jurkat cells were treated and stained with Cell Meter™ BX590 (Cat#22514), and then fixed in 3.7% formaldehyde and analyzed by flow cytometry.  The dead cell population (Blue peak)  is easily distinguished from the live cell population (Red peak)  with PE-TexasRed channel, and nearly identical results were obtained before and after fixation.

Protocol

SDS

Catalog	Size	Price	Quantity
22514	200 Tests	Price

Overview

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations. Cell Meter™ fixable viability dyes are a large family of cell-impermeable fluorescent viability dyes that are optimized to match the major excitation lasers of common flow cytometers, such as 350, 405, 488, 633 and 647 nm. These dyes are impermeant to live cells but permeant to cells with compromised membranes. They irreversibly react with amine- and thiol-containing proteins and other cellular components. Since dead or fixed cells with a compromised membrane more readily react with Cell Meter™ fixable cell stains, thus stain brighter than live cells with an intact membrane, these dyes can be used to assess live vs. dead status of mammalian cells. There are a few factors to be considered when using these dyes, e.g., the titration of each dye to ensure that live cells have minimal to no staining. Cell Meter™ BX590 fixable cell stain is optimized to be excited with the blue laser at 488 nm with emission at 590 nm. Compared to other commercially similar viability dyes, this fixable viability dye is much more robust and stable.

References

View all 50 references: Citation Explorer

CFSE dilution to study human T and NK cell proliferation in vitro.

Authors:

Terrén, Iñigo and Orrantia, Ane and Vitallé, Joana and Zenarruzabeitia, Olatz and Borrego, Francisco

Journal:

Methods in enzymology (2020): 239-255

Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells.

Authors:

Azari, Hassan and Deleyrolle, Loic P and Reynolds, Brent A

Journal:

Methods in molecular biology (Clifton, N.J.) (2018): 59-67

Estimates and impact of lymphocyte division parameters from CFSE data using mathematical modelling.

Authors:

Mazzocco, Pauline and Bernard, Samuel and Pujo-Menjouet, Laurent

Journal:

PloS one (2017): e0179768

Assessment of lymphocyte proliferation for diagnostic purpose: Comparison of CFSE staining, Ki-67 expression and 3H-thymidine incorporation.

Authors:

Lašťovička, Jan and Rataj, Michal and Bartůňková, Jiřina

Journal:

Human immunology (2016): 1215-1222

Analysis of CFSE time-series data using division-, age- and label-structured population models.

Authors:

Hross, Sabrina and Hasenauer, Jan

Journal:

Bioinformatics (Oxford, England) (2016): 2321-9

Physical properties

Solvent

DMSO

Spectral properties

Absorbance (nm)	492
Excitation (nm)	492
Emission (nm)	579

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	575/26 nm filter
Instrument specification(s)	PE channel

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on November 15, 2026