Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 75000 |
Excitation (nm) | 503 |
Emission (nm) | 525 |
Quantum yield | 0.9 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Alternative formats
Related products
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See also: Caspases
Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 75000 | Excitation (nm) 503 | Emission (nm) 525 | Quantum yield 0.9 |
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring generic caspases 3/7 activation in living cells. Caspase 3/7 activation is widely accepted as a reliable indicator for cell apoptosis. Caspases have substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses TF2-DEVD-FMK as a fluorogenic indicator for caspase 3/7 activity. TF2-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated casepase 3/7 in apoptotic cells. Once bound to caspases, the red fluorescent reagent is retained within the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. The reagent will start to react with active caspase enzymes within 15 minutes of addition to the media. The kit provides all the essential components with an optimized assay protocol. It is used for the quantification of most activated caspases activities in apoptotic cells, or for screening caspases inhibitors. The green label allows for direct detection of activated caspases in apoptotic cells by flow cytometry.
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 530/30 nm filter |
Instrument specification(s) | FITC channel |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells with test compounds at a density of 5 × 105 to 1 × 106 cells/mL
- Add 1 µL of 500X TF2-DEVD-FMK into 0.5 mL of cell solution
- Incubate at 37°C, 5% CO2 incubator for 1 - 4 hours
- Pellet the cells and resuspend the cells in 0.5 mL of assay buffer or growth medium
- Analyze cells using flow cytometer with 530/30 nm filter (FITC channel)
Important notes
Thaw all the components at room temperature before starting the experiment.
SAMPLE EXPERIMENTAL PROTOCOL
- For each sample, prepare cells in 0.5 mL warm medium or buffer of your choice at a density of 5×105 to 1×106 cells/mL. Note: Each cell line should be evaluated on an individual basis to determine the optimal cell density for apoptosis induction.
- Treat cells with test compounds for a desired period of time to induce apoptosis, and create positive and negative controls.
- Add 1 µL of 500X TF2-DEVD-FMK (Component A) into the treated cells.
- Incubate the cells in a 37°C, 5% CO2 incubator for 1 - 4 hours. Note: For adherent cells, gently lift the cells with 0.5 mM EDTA to keep the cells intact, and wash the cells once with serum-containing media prior to incubation with TF2-DEVD-FMK. The appropriate incubation time depends on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.
- Wash and spin the cells twice. Resuspend the cells in 0.5 mL of assay buffer or growth medium. Note: TF2-DEVD-FMK is fluorescent; theforefore it is important to wash out any unbound reagent to remove the background.
- If desired, label the cells with a DNA stain (such as propidium iodide or 7-AAD for dead cells).
- If desired, fix cells.
- Monitor the fluorescence intensity using a flow cytometer with 530/30 nm filter (FITC channel). Gate on the cells of interest, excluding debris.
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 75000 |
Excitation (nm) | 503 |
Emission (nm) | 525 |
Quantum yield | 0.9 |
Images

Figure 1. Detection of caspase 3/7 activities using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit in Jurkat cells. TF2-DEVD-FMK fluorescence intensity was induced with the addition of camptothecin. Jurkat cells were treated without (Blue) or with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with TF2-DEVD-FMK for 1 hour. Response was measured using BD FACSCalibur using FL1 channel.
Citations
View all 36 citations: Citation Explorer
Trans-[bis (benzimidazol-2-ylidene) dichlorido] platinum (II) complexes with peculiar modes of action and activity against cisplatin-resistant cancer cells
Authors: B{\"a}r, Sofia I and Schleser, Sebastian W and Oberhuber, Natalie and Herrmann, Alexander and Schlotte, Luca and Weber, Stefanie E and Schobert, Rainer
Journal: Journal of Inorganic Biochemistry (2023): 112028
Authors: B{\"a}r, Sofia I and Schleser, Sebastian W and Oberhuber, Natalie and Herrmann, Alexander and Schlotte, Luca and Weber, Stefanie E and Schobert, Rainer
Journal: Journal of Inorganic Biochemistry (2023): 112028
Metformin sensitizes AML cells to venetoclax through endoplasmic reticulum stress—CHOP pathway
Authors: Hua, Lei and Yang, Nianhui and Li, Yue and Huang, Kexiu and Jiang, Xinya and Liu, Fangshu and Yu, Zhi and Chen, Jie and Lai, Jing and Du, Juan and others,
Journal: British Journal of Haematology (2023)
Authors: Hua, Lei and Yang, Nianhui and Li, Yue and Huang, Kexiu and Jiang, Xinya and Liu, Fangshu and Yu, Zhi and Chen, Jie and Lai, Jing and Du, Juan and others,
Journal: British Journal of Haematology (2023)
Apical-Out Chicken Enteroids as an In-Vitro Model to Study Effects of an Alternative Growth Promoter on Indicators of Gut Health
Authors: Tamazian, Lorik
Journal: (2023)
Authors: Tamazian, Lorik
Journal: (2023)
GONIOTHALAMIN INHIBITS CELL GROWTH, PERTURBS CELL CYCLE AND INDUCES APOPTOSIS IN HUMAN OSTEOSARCOMA SAOS-2 CELLS: Received 2023-01-03; Accepted 2023-02-15; Published 2023-06-06
Authors: Bakar, Siti Aishah Abu and Azhar, Nur Asna and Mohamad, Noor Muzamil and Nordin, Ira Maya Sophia and Ali, Abdul Manaf and Noor, Siti Noor Fazliah Mohd and Hamid, Shahrul Bariyah Sahul and Ahmad, Nor Hazwani
Journal: Journal of Health and Translational Medicine (JUMMEC) (2023): 105--115
Authors: Bakar, Siti Aishah Abu and Azhar, Nur Asna and Mohamad, Noor Muzamil and Nordin, Ira Maya Sophia and Ali, Abdul Manaf and Noor, Siti Noor Fazliah Mohd and Hamid, Shahrul Bariyah Sahul and Ahmad, Nor Hazwani
Journal: Journal of Health and Translational Medicine (JUMMEC) (2023): 105--115
Mahuang Xixin Fuzi decoction ameliorates apoptosis via the mitochondrial-mediated signaling pathway in MCM cells
Authors: Yang, Jia and Sun, Qihui and Qingyun, MA and Yu, Qinhui and Liu, Xiaoyun and Liu, Yanliang and Han, Yuxiu and Yang, Yong and Rong, Rong
Journal: Journal of Ethnopharmacology (2022): 115538
Authors: Yang, Jia and Sun, Qihui and Qingyun, MA and Yu, Qinhui and Liu, Xiaoyun and Liu, Yanliang and Han, Yuxiu and Yang, Yong and Rong, Rong
Journal: Journal of Ethnopharmacology (2022): 115538
Oxime derivative TFOBO promotes cell death by modulating reactive oxygen species and regulating NADPH oxidase activity in myeloid leukemia
Authors: Jo, Ahyoung and Kwak, Jae-Hwan and Woo, Soo-Yeon and Kim, Bo-Young and Son, Yonghae and Choi, Hee-Seon and Kim, Jayoung and Kwon, Munju and Cho, Hyok-Rae and Eo, Seong-Kug and others,
Journal: Scientific reports (2022): 1--10
Authors: Jo, Ahyoung and Kwak, Jae-Hwan and Woo, Soo-Yeon and Kim, Bo-Young and Son, Yonghae and Choi, Hee-Seon and Kim, Jayoung and Kwon, Munju and Cho, Hyok-Rae and Eo, Seong-Kug and others,
Journal: Scientific reports (2022): 1--10
Revisiting the anticancer properties of phosphane (9-ribosylpurine-6-thiolato) gold (I) complexes and their 9H-purine precursors
Authors: Kober, Luisa and Schleser, Sebastian W and B{\"a}r, Sofia I and Schobert, Rainer
Journal: JBIC Journal of Biological Inorganic Chemistry (2022): 731--745
Authors: Kober, Luisa and Schleser, Sebastian W and B{\"a}r, Sofia I and Schobert, Rainer
Journal: JBIC Journal of Biological Inorganic Chemistry (2022): 731--745
Visfatin inhibits colon cancer cell apoptosis and decreases chemosensitivity to 5-FU by promoting the SDF-1/CXCR4/Akt axis
Authors: Zhao, Quan and Long, Yaxin and Cheng, Wen and Huang, Yingguang and Li, Jinyuan and Li, Yuejin and Li, Xing and Guo, Xiaodong and Li, Yu and Li, Guosan and others,
Journal: International journal of oncology (2022): 1--13
Authors: Zhao, Quan and Long, Yaxin and Cheng, Wen and Huang, Yingguang and Li, Jinyuan and Li, Yuejin and Li, Xing and Guo, Xiaodong and Li, Yu and Li, Guosan and others,
Journal: International journal of oncology (2022): 1--13
Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7
Authors: Bakar, Siti Aishah Abu and Ali, Abdul Manaf and Noor, Siti Noor Fazliah Mohd and Hamid, Shahrul Bariyah Sahul and Azhar, Nur Asna and Mohamad, Noor Muzamil and Ahmad, Nor Hazwani
Journal: BioMed Research International (2022)
Authors: Bakar, Siti Aishah Abu and Ali, Abdul Manaf and Noor, Siti Noor Fazliah Mohd and Hamid, Shahrul Bariyah Sahul and Azhar, Nur Asna and Mohamad, Noor Muzamil and Ahmad, Nor Hazwani
Journal: BioMed Research International (2022)
The BH3 mimetic ($\pm$) gossypol induces ROS-independent apoptosis and mitochondrial dysfunction in human A375 melanoma cells in vitro
Authors: Haasler, Lisa and Kondadi, Arun Kumar and Tsigaras, Thanos and von Montfort, Claudia and Graf, Peter and Stahl, Wilhelm and Brenneisen, Peter
Journal: Archives of Toxicology (2021): 1349--1365
Authors: Haasler, Lisa and Kondadi, Arun Kumar and Tsigaras, Thanos and von Montfort, Claudia and Graf, Peter and Stahl, Wilhelm and Brenneisen, Peter
Journal: Archives of Toxicology (2021): 1349--1365
Application notes
FAQ
How do I choose the best method to assess cell survival?
Are inflammasomes and caspase-1 related?
Do you offer any fluorimetric assays that measure caspase activation/activity in live cells using a flow cytometer?
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Does propidium iodide stain apoptotic cells?
Are inflammasomes and caspase-1 related?
Do you offer any fluorimetric assays that measure caspase activation/activity in live cells using a flow cytometer?
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Does propidium iodide stain apoptotic cells?