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Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit
Green Fluorescence Optimized for Flow Cytometry

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis by measuring generic caspase 3/7 activation in living cells. Caspase 3/7 activation is widely accepted as a reliable indicator for cell apoptosis. Caspases have substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses TF2-DEVD-FMK as a fluorogenic indicator for caspase 3/7 activity. TF2-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 3/7 in apoptotic cells. Once bound to caspases, the green fluorescent reagent is retained within the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. The reagent will start to react with active caspase enzymes within 15 minutes of addition to the media. The kit provides all the essential components with an optimized assay protocol. It is used for the quantification of most activated caspase activities in apoptotic cells, or for screening caspase inhibitors. The green label allows for direct detection of activated caspases in apoptotic cells by flow cytometry.

Detection of caspase 3/7 activities using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit in Jurkat cells. TF2-DEVD-FMK fluorescence intensity was induced with the addition of camptothecin. Jurkat cells were treated without (Blue) or with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with TF2-DEVD-FMK for 1 hour. Response was measured using BD FACSCalibur using FL1 channel.
Detection of caspase 3/7 activities using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit in Jurkat cells. TF2-DEVD-FMK fluorescence intensity was induced with the addition of camptothecin. Jurkat cells were treated without (Blue) or with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with TF2-DEVD-FMK for 1 hour. Response was measured using BD FACSCalibur using FL1 channel.
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Price
Quantity
22823100 Tests
Price
 
Spectral properties

Correction factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)
75000
Excitation (nm)503
Emission (nm)525
Quantum yield
0.9
1
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
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Page updated on September 24, 2025