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Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit
Red Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for cell apoptosis since the activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses Z-DEVD-ProRed™ as the fluorogenic indicator for caspase-3 activity. Cleavage of ProRed™ DEVD blocking peptide residue by caspase 3 generates strongly red fluorescent ProRed™ that is monitored fluorimetrically at ~620 nm with excitation of ~530 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays. Using 100 uL of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 100 assays. Using 25 uL of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 400 assays.
Detection of Caspase 3/7 Activities in Jurkat cells. Jurkat cells were seeded on the same day at 200,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1 µM of staurosporine for 5 hours. The caspase 3/7 working solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 540/620 nm (Cutoff = 610 nm) with FlexStation fluorescence microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
22797100 Tests
Price
 
Spectral properties
Excitation (nm)532
Emission (nm)619
Usage and storage
Intended useResearch Use Only (RUO)
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission620 nm
Cutoff610 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Top or bottom read mode
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Page updated on July 25, 2026