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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit
Blue Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions. There are a variety of parameters that can be used. This particular kit is designed to monitor cell apoptosis by measuring caspase 9 activity. Caspase 9 is a member of the CED-3 subfamily. Activated Caspase-9 cleaves downstream caspases such as caspase-3, -6 and -7, initiating the caspase cascade. It is essential for apoptosis during normal development of the central nervous system. Caspase 9 is proven to have selectivity for the peptide sequence Leu-Glu-His-Asp (LEHD). This kit uses Ac-LEHD-AMC as a fluorogenic indicator for caspase 9 activity. Cleavage of AMC by caspase 9 generates strongly fluorescent AMC. The kit provides all the essential components. The assay is robust and can be readily adapted for high throughput screening. It can be used to either quantify the activated caspase 9 activities in apoptotic cells or screen the caspase 9 inhibitors. Quite a few labs have used this kit for high throughput screenings.
Detection of Caspase 9 Activities in Jurkat cells using Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*. Jurkat cells were seeded at 300,000 cells/90 μL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1μM of staurosporine for 4 hours. The caspase 9 Substrate working solution (100 μL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 375/435 nm  (Cutoff = 420 nm) with a FlexStation™ microplate reader (Molecular Devices).
Detection of Caspase 9 Activities in Jurkat cells using Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*. Jurkat cells were seeded at 300,000 cells/90 μL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1μM of staurosporine for 4 hours. The caspase 9 Substrate working solution (100 μL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 375/435 nm  (Cutoff = 420 nm) with a FlexStation™ microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22813200 Tests
Price
 
Spectral properties
Excitation (nm)341
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation375 nm
Emission435 nm
Cutoff420 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Top/Bottom read mode
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Page updated on September 25, 2025