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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit
Red Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions. There are a variety of parameters that can be used. This particular kit is designed to monitor cell apoptosis by measuring caspase 9 activity. Caspase 9 is a member of the CED-3 subfamily. Activated Caspase-9 cleaves downstream caspases such as caspase-3, -6 and -7, initiating the caspase cascade. It is essential for apoptosis during normal development of the central nervous system. Caspase 9 is proven to have selectivity for the peptide sequence Leu-Glu-His-Asp (LEHD). This kit uses Ac-LEHD-ProRed™ as a fluorogenic indicator for caspase 9 activity. Cleavage of ProRed™ by caspase 9 generates strongly fluorescent ProRed™. The kit provides all the essential components. The assay is robust and can be readily adapted for high throughput screening. It can be used to either quantify the activated caspase 9 activities in apoptotic cells or screen the caspase 9 inhibitors. Quite a few labs have used this kit for high throughput screenings.
Detection of Caspase 9 Activities in Jurkat cells using Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*. Jurkat cells were seeded on the same day at 300,000 cells/90 µL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1 µM of staurosporine for 4 hours. The caspase 9 Substrate working solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 540/620 nm (Cutoff = 610 nm) with FlexStation fluorescence microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
22817100 Tests
Price
 
Spectral properties
Excitation (nm)532
Emission (nm)619
Usage and storage
Intended useResearch Use Only (RUO)
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission620 nm
Cutoff610 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Top or bottom read mode
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Page updated on July 16, 2026