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Cell Meter™ Cell Proliferation Assay Kit

Quantification of HeLa cells using Cell Meter™ Cell Proliferation Assay kit. The linear range of cells from 50 to 50,000 HeLa cells was plated and assay was performed as per protocol. The insert shows the linearity that can be obtained at very low number of cells.
Quantification of HeLa cells using Cell Meter™ Cell Proliferation Assay kit. The linear range of cells from 50 to 50,000 HeLa cells was plated and assay was performed as per protocol. The insert shows the linearity that can be obtained at very low number of cells.
Ordering information
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Catalog Number22505
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Spectral properties
Excitation (nm)503
Emission (nm)527
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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OverviewpdfSDSpdfProtocol


Excitation (nm)
503
Emission (nm)
527
Cell Meter™ Cell Proliferation Assay Kit is a sensitive fluorescence-based method for quantifying cells and assessing cell proliferation and cytotoxicity in a microplate format. The kit uses our Nuclear Green LCS1, a proprietary fluorescent dye that exhibits strong fluorescence upon binding to nucleic acids. The use of Nuclear Green LCS1 improves accuracy over cell metabolism-based cell proliferation or cytotoxicity assays that can be influenced by cell changes that are unrelated to cell numbers. The kit does not require cell lysis, long incubations or removal of cells with minimal hands-on time. It is compatible with high throughput screening.

Platform


Fluorescence microplate reader

Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom

Components


Nuclear Green™ LCS11 vial (50 µL)
Reaction Buffer1 bottle (20 mL)

Example protocol


AT A GLANCE

Protocol Summary
  1. Prepare the cell samples and treat cells as desired

  2. Remove cell culture medium

  3. Add dye working solution
  4. Incubate for 10 minutes

  5. Analyze with fluorescence microplate reader using Ex/Em = 490/525 nm

Important: Allow all the components to warm to room temperature before opening the vials. Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues. 

PREPARATION OF WORKING SOLUTION

Nuclear Green™ LCS1 dye working solution

Add 25 µL of Nuclear Green™ LCS1 to 10 mL of Reaction Buffer and mix well.

Note: 10 mL volume is sufficient for 100 tests. For best results, the Nuclear Green™ LCS1 dye working solution should be used promptly and in its entirety. 

Note: Store any unused Nuclear Green™ LCS1 (Component A) at -20 °C.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used for guidelines.

  1. Prepare the cell samples and treat cells as desired.

  2. Remove the cell culture medium and wash twice with PBS.

    Note: Removal of cell culture medium is necessary since it may interfere with the fluorescence of Nuclear Green™ LCS1.

  3. Optional: After washing with PBS, experiments involving multiple time points and/or samples can be stored at -80 °C for a few days or proceed to step 4.

  4. Add 100 µL of Nuclear Green™ LCS1 dye working solution and incubate for 10 to 60 minutes at RT, protected from light.

  5. Measure the sample fluorescence using a fluorescence microplate reader with Ex/Em = 490/525 nm (Cutoff = 515 nm).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Excitation (nm)503
Emission (nm)527

References


View all 50 references: Citation Explorer
[Retracted] MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3.
Authors: Liu, Nian and Liu, Zhiguang and Zhang, Weidong and Li, Yang and Cao, Jun and Yang, Huan and Li, Xiuying
Journal: Molecular medicine reports (2022)
Dantrolene reduces platelet-derived growth factor (PDGF)-induced vascular smooth muscle cell proliferation and neointimal formation following vascular injury in mice.
Authors: Sakai, Chihiro and Mikawa, Mei and Yamamoto, Takeshi and Uchida, Tomoyuki and Nakamura, Yoshihide and Akase, Hideaki and Suetomi, Takeshi and Tominaga, Naoomi and Inamitsu, Masako and Oda, Tetsuro and Okamura, Takayuki and Kobayashi, Shigeki and Yano, Masafumi
Journal: Biochemical and biophysical research communications (2022): 51-58
MiR-140 targets lncRNA FAM230B to suppress cell proliferation in acute myeloid leukemia running title: MiR-140 targets FAM230B in AML.
Authors: Wang, Yan and Wang, Fangfang and Lu, Yang and Li, Yan and Ran, Haonan and Yan, Feihu and Tian, Yuyang
Journal: Hematology (Amsterdam, Netherlands) (2022): 700-705
Early T-cell precursor lymphoblastic leukemia accompanied by prominent blastic plasmacytoid dendritic cell proliferation mimicking blastic plasmacytoid dendritic cell neoplasm: an exceptional case report and literature review.
Authors: Liao, Hongyan and Yu, Jiang and Liu, Yu and Zhao, Sha and Zhu, Huanling and Xu, Dongsheng and Jiang, Nenggang and Zheng, Qin
Journal: Journal of cancer research and clinical oncology (2022): 2911-2919
G protein-coupled receptor 30 mediates cell proliferation of goat mammary epithelial cells via MEK/ERK&PI3K/AKT signaling pathway.
Authors: Zhao, Ying and Liu, Haokun and Fan, Mingzhen and Miao, Yuyang and Zhao, Xiaoe and Wei, Qiang and Ma, Baohua
Journal: Cell cycle (Georgetown, Tex.) (2022): 2027-2037
Human dental pulp cells modulate CD8+ T cell proliferation and efficiently degrade extracellular ATP to adenosine in vitro.
Authors: Ahmadi, Parimah and Yan, Ming and Bauche, Andreas and Smeets, Ralf and Müller, Christa E and Koch-Nolte, Friedrich and Haag, Friedrich and Fliegert, Ralf and Kluwe, Lan and Schulze Zur Wiesch, Julian and Hartjen, Philip
Journal: Cellular immunology (2022): 104589
Reply to "Comments on: Acute aerobic exercise-conditioned serum reduces colon cancer cell proliferation in vitro through interleukin-6-induced regulation of DNA damage".
Authors: Orange, Samuel T and Jordan, Alastair R and Odell, Adam and Kavanagh, Owen and Hicks, Kirsty M and Todryk, Stephen and Saxton, John M
Journal: International journal of cancer (2022): 1642-1643
CPNE1 silencing inhibits cell proliferation and accelerates apoptosis in human gastric cancer.
Authors: Li, Yan and Li, Lixiang and Liu, Han and Zhou, Tao
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2022): 106278
EdU sensing: The Raman way of following endothelial cell proliferation in vitro and ex vivo.
Authors: Radwan, Basseem and Rocchetti, Stefano and Matuszyk, Ewelina and Sternak, Magdalena and Stodulski, Maciej and Pawlowski, Robert and Mlynarski, Jacek and Brzozowski, Krzysztof and Chlopicki, Stefan and Baranska, Malgorzata
Journal: Biosensors & bioelectronics (2022): 114624
SPRY1 promotes cell proliferation and inhibits apoptosis by activating Hedgehog pathway in acute myeloid leukemia.
Authors: Lv, Guiyang and Wang, Yuanyuan and Ji, ChunXiao and Shi, Chunlei and Li, Ying
Journal: Hematology (Amsterdam, Netherlands) (2022): 1-10