Cell Meter™ Cell Proliferation Assay Kit
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Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Excitation (nm) | 503 |
Emission (nm) | 527 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | ![]() ![]() |
Excitation (nm) 503 | Emission (nm) 527 |
Platform
Fluorescence microplate reader
Excitation | 490 nm |
Emission | 525 nm |
Cutoff | 515 nm |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Prepare the cell samples and treat cells as desired
Remove cell culture medium
- Add dye working solution
Incubate for 10 minutes
Analyze with fluorescence microplate reader using Ex/Em = 490/525 nm
Important: Allow all the components to warm to room temperature before opening the vials. Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues.
PREPARATION OF WORKING SOLUTION
Add 25 µL of Nuclear Green™ LCS1 to 10 mL of Reaction Buffer and mix well.
Note: 10 mL volume is sufficient for 100 tests. For best results, the Nuclear Green™ LCS1 dye working solution should be used promptly and in its entirety.
Note: Store any unused Nuclear Green™ LCS1 (Component A) at -20 °C.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be used for guidelines.
Prepare the cell samples and treat cells as desired.
Remove the cell culture medium and wash twice with PBS.
Note: Removal of cell culture medium is necessary since it may interfere with the fluorescence of Nuclear Green™ LCS1.
Optional: After washing with PBS, experiments involving multiple time points and/or samples can be stored at -80 °C for a few days or proceed to step 4.
Add 100 µL of Nuclear Green™ LCS1 dye working solution and incubate for 10 to 60 minutes at RT, protected from light.
Measure the sample fluorescence using a fluorescence microplate reader with Ex/Em = 490/525 nm (Cutoff = 515 nm).
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence with 405 nm Excitation* | 406 | 445 |
Cell Meter™ Cell Viability Assay Kit *Green Fluorescence* | 494 | 514 |
Cell Meter™ Cell Adhesion Assay Kit | 494 | 514 |
Images
References
Authors: Liu, Nian and Liu, Zhiguang and Zhang, Weidong and Li, Yang and Cao, Jun and Yang, Huan and Li, Xiuying
Journal: Molecular medicine reports (2022)
Authors: Sakai, Chihiro and Mikawa, Mei and Yamamoto, Takeshi and Uchida, Tomoyuki and Nakamura, Yoshihide and Akase, Hideaki and Suetomi, Takeshi and Tominaga, Naoomi and Inamitsu, Masako and Oda, Tetsuro and Okamura, Takayuki and Kobayashi, Shigeki and Yano, Masafumi
Journal: Biochemical and biophysical research communications (2022): 51-58
Authors: Wang, Yan and Wang, Fangfang and Lu, Yang and Li, Yan and Ran, Haonan and Yan, Feihu and Tian, Yuyang
Journal: Hematology (Amsterdam, Netherlands) (2022): 700-705
Authors: Liao, Hongyan and Yu, Jiang and Liu, Yu and Zhao, Sha and Zhu, Huanling and Xu, Dongsheng and Jiang, Nenggang and Zheng, Qin
Journal: Journal of cancer research and clinical oncology (2022): 2911-2919
Authors: Zhao, Ying and Liu, Haokun and Fan, Mingzhen and Miao, Yuyang and Zhao, Xiaoe and Wei, Qiang and Ma, Baohua
Journal: Cell cycle (Georgetown, Tex.) (2022): 2027-2037
Authors: Ahmadi, Parimah and Yan, Ming and Bauche, Andreas and Smeets, Ralf and Müller, Christa E and Koch-Nolte, Friedrich and Haag, Friedrich and Fliegert, Ralf and Kluwe, Lan and Schulze Zur Wiesch, Julian and Hartjen, Philip
Journal: Cellular immunology (2022): 104589
Authors: Orange, Samuel T and Jordan, Alastair R and Odell, Adam and Kavanagh, Owen and Hicks, Kirsty M and Todryk, Stephen and Saxton, John M
Journal: International journal of cancer (2022): 1642-1643
Authors: Li, Yan and Li, Lixiang and Liu, Han and Zhou, Tao
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2022): 106278
Authors: Radwan, Basseem and Rocchetti, Stefano and Matuszyk, Ewelina and Sternak, Magdalena and Stodulski, Maciej and Pawlowski, Robert and Mlynarski, Jacek and Brzozowski, Krzysztof and Chlopicki, Stefan and Baranska, Malgorzata
Journal: Biosensors & bioelectronics (2022): 114624
Authors: Lv, Guiyang and Wang, Yuanyuan and Ji, ChunXiao and Shi, Chunlei and Li, Ying
Journal: Hematology (Amsterdam, Netherlands) (2022): 1-10