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Cell Meter™ Cell Viability Assay Kit
Blue Fluorescence with 405 nm Excitation
There are a variety of parameters that can be used to monitor cell viability. The proprietary violet laser -excitable fluorescent dye used in the kit is a hydrophobic compound that easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly blue fluorescent product that is well-retained in the cell cytoplasm. The blue fluorophore generated by the esterase hydrolysis of the non-fluorescent substrate has the spectral properties of fluorescein. When excited at 405 nm, the fluoreophore emits intense blue fluorescence at ~450 nm. The kit provides all the essential components with an optimized cell-labeling protocol for fluorescence microplate assays. This Cell Meter™ Cell Viability Assay Kit provides an effective tool of labeling cells for fluorescence flow cytometry, microplate and microscopic investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit is suitable for proliferating and non-proliferating cells.
CHO-K1 cell number response was measured with Cell Meter&trade; Cell Viability Assay Kit. CHO-K1 cells at 0 to 5,000 cells/well/100 &micro;L were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 &micro;L/well of CytoCalcein&trade; Violet 450, AM dye-working solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 405/460 nm with NOVOstar instrument (from BMG Labtech). The fluorescence intensity was linear (R<sup>2</sup> = 1) to the cell number as indicated.
CHO-K1 cell number response was measured with Cell Meter&trade; Cell Viability Assay Kit. CHO-K1 cells at 0 to 5,000 cells/well/100 &micro;L were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 &micro;L/well of CytoCalcein&trade; Violet 450, AM dye-working solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 405/460 nm with NOVOstar instrument (from BMG Labtech). The fluorescence intensity was linear (R<sup>2</sup> = 1) to the cell number as indicated.
CatalogSize
Price
Quantity
22784500 Tests
Price
 
Spectral properties

Excitation (nm)406
Emission (nm)445
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation405 nm
Emission460 nm
Cutoff435 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 9, 2025