Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence with 405 nm Excitation*

Cell Meter™ Cell Viability Assay Kit

Blue Fluorescence with 405 nm Excitation

There are a variety of parameters that can be used to monitor cell viability. The proprietary violet laser -excitable fluorescent dye used in the kit is a hydrophobic compound that easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly blue fluorescent product that is well-retained in the cell cytoplasm. The blue fluorophore generated by the esterase hydrolysis of the non-fluorescent substrate has the spectral properties of fluorescein. When excited at 405 nm, the fluoreophore emits intense blue fluorescence at ~450 nm. The kit provides all the essential components with an optimized cell-labeling protocol for fluorescence microplate assays. This Cell Meter™ Cell Viability Assay Kit provides an effective tool of labeling cells for fluorescence flow cytometry, microplate and microscopic investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit is suitable for proliferating and non-proliferating cells.

CHO-K1 cell number response was measured with Cell Meter™ Cell Viability Assay Kit. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of CytoCalcein™ Violet 450, AM dye-working solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 405/460 nm with NOVOstar instrument (from BMG Labtech). The fluorescence intensity was linear (R<sup>2</sup> = 1) to the cell number as indicated.

Protocol

SDS

Catalog	Size	Price	Quantity
22784	500 Tests	Price

Citations

View all 18 citations: Citation Explorer

Regulation of high glucose-induced apoptosis of brain pericytes by mitochondrial CA VA: A specific target for prevention of diabetic cerebrovascular pathology

Authors:

Price, Tulin O and Sheibani, Nader and Shah, Gul N

Journal:

Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2017): 929--935

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice

Authors:

Li, Jingcheng and Zhang, Jianxiong and Wang, Meng and Pan, Junxia and Chen, Xiaowei and Liao, Xiang

Journal:

Biomedical Optics Express (2017): 2599--2610

NINJ2--A novel regulator of endothelial inflammation and activation

Authors:

Wang, Jingjing and Fa, Jingjing and Wang, Pengyun and Jia, Xinzhen and Peng, Huixin and Chen, Jing and Wang, Yifan and Wang, Chenhui and Chen, Qiuyun and Tu, Xin and others, undefined

Journal:

Cellular Signalling (2017)

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B

Authors:

Bogert, Nicolai V and Werner, Isabella and Kornberger, Angela and Meybohm, Patrick and Moritz, Anton and Keller, Till and Stock, Ulrich A and Beiras-Fern, undefined and ez, Andres

Journal:

Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water

Authors:

Alharbi, Hattan A and Saunders, David MV and Al-Mousa, Ahmed and Alcorn, Jane and Pereira, Alberto S and Martin, Jonathan W and Giesy, John P and Wiseman, Steve B

Journal:

Aquatic Toxicology (2016): 81--88

References

View all 84 references: Citation Explorer

Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development

Authors:

Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang

Journal:

Biochemical and Biophysical Research Communications (2017)

Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine

Authors:

Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.

Journal:

Biophys J. (2006)

A vaccination and challenge model using calcein marked fish

Authors:

Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.

Journal:

Fish Shellfish Immunol (2006): 20

Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging

Authors:

Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.

Journal:

Cytometry A (2005): 78

Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake

Authors:

Schoonen WG, Westerink WM, de Roos JA, Debiton E.

Journal:

Toxicol In Vitro (2005): 505

Spectral properties

Excitation (nm)	406
Emission (nm)	445

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	405 nm
Emission	460 nm
Cutoff	435 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Cell Viability & Proliferation

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2025

Component A: CytoCalcein™ Violet 450, AM	5 vials, lyophilized
Component B: DMSO	1 vial (200 µL)
Component C: Assay Buffer	1 bottle (50 mL)