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Cell Meter™ Cell Viability Assay Kit
Blue Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This kit uses a proprietary dye that gets enhanced fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells, and thus directly related to the fluorescence intensity of the product generated from the esterase-catalyzed hydrolysis of the fluorogenic substrate. Cells grown in black-walled plates can be stained and quantified in less than two hours. The assay is more robust than the tetrazolium salt or Alarmar Blue™-based assays. It can be readily adapted for high-throughput assays in a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. Using 100 uL of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 500 assays. Using 25 uL of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 2,000 assays.
CHO-K1 cell number response was measured with Cell Meter&trade; Cell Viability Assay Kit. CHO-K1 cells at 0 to 5,000 cells/well/100 &micro;L were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 &micro;L/well of CytoCalcein Blue, AM dye-working solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 360/450 nm with NOVOstar instrument (from BMG Labtech). The fluorescence intensity was linear (R<sup>2</sup>=1) to the cell number as indicated.
CHO-K1 cell number response was measured with Cell Meter&trade; Cell Viability Assay Kit. CHO-K1 cells at 0 to 5,000 cells/well/100 &micro;L were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 &micro;L/well of CytoCalcein Blue, AM dye-working solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 360/450 nm with NOVOstar instrument (from BMG Labtech). The fluorescence intensity was linear (R<sup>2</sup>=1) to the cell number as indicated.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22785500 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation360 nm
Emission450 nm
Cutoff420 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on September 29, 2025