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Cell Meter™ Colorimetric Cell Cytotoxicity Assay Kit
Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions. Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This kit uses a proprietary water-soluble and cell-permeable dye that changes its absorption spectra upon cellular reduction. The absorption ratio change is directly proportional to the number of living cells. The characteristics of its high sensitivity, non-radioactivity and no-wash method make the kit suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds. This kit does not require pre-mixing of components and has higher sensitivity compared to the traditional tetrazolium-based colorimetric assays (such as WST-8, MTT and XTT). The kit provides the reagents sufficient to run 1000 assays (regular size) or 5000 assays (bulk package). All the kit components are quite stable with minimal cytotoxicity, thus a longer incubation time (such as 24 to 48 hours) is possible if required. Our Cell Meter™ Colorimetric Cell Cytoxicity Assay Kit is robust and convenient to use. It can be readily adapted for a wide variety of instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
CHO-K1 cell number response was measured with Cell Meter™ Colorimetric Cell Cytotoxicity Assay Kit. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96- well plate. The cells were incubated with 20 µL/well of Assay Solution (Component A) for 3 hours at 37 °C. The absorbance intensity was measured at 570 nm and 605 nm with SpectraMax plus (Molecular Devices). The ratio of OD570/OD605 is proportional to the number of cells as indicated.
CHO-K1 cell number response was measured with Cell Meter™ Colorimetric Cell Cytotoxicity Assay Kit. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a Costar black wall/clear bottom 96- well plate. The cells were incubated with 20 µL/well of Assay Solution (Component A) for 3 hours at 37 °C. The absorbance intensity was measured at 570 nm and 605 nm with SpectraMax plus (Molecular Devices). The ratio of OD570/OD605 is proportional to the number of cells as indicated.
protocol
Protocol
COA
CatalogSize
Price
Quantity
227795000 Tests
Price
227801000 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance570/605 nm
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet (Catalog 22780)
Safety Data Sheet (Catalog 22779)
Certificate of Analysis
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Page updated on October 21, 2025