Cell Meter™ Colorimetric MTS Cell Proliferation Kit

Cell Meter™ Colorimetric MTS Cell Proliferation Kit enables convenient, high-throughput measurement of cell viability and proliferation using a soluble formazan-based colorimetric assay.

Ready-to-use format: Convenient ready-to-use formulation simplifies cell viability and proliferation assays
High-throughput capability: Optimized for large-scale screening of cell proliferation and cytotoxicity in 96-well formats
Application versatility: Suitable for evaluating effects of growth factors, drugs, toxins, and other stimuli on cell viability
Comparable alternative: Provides a reliable solution comparable to Promega's CellTiter 96® AQueous One Solution Cell Proliferation Assay

Cell count measured using the Cell Meter™ Colorimetric MTS Cell Quantification Kit. HeLa cells ranging from 781 to 50,000 cells/well/100 µL were added to a clear bottom 96-well plate. After incubation with the MTS solution for 4 hours, the absorbance was measured at 490 nm using a ClarioStar absorbance microplate reader.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22766	1000 tests	Price
22767	5000 tests	Price

Overview

The Cell Meter™ Colorimetric MTS Cell Proliferation Kit is a quick and reliable method for quantifying viable cells in proliferation and cytotoxicity assays. This technique relies on the reduction of the MTS tetrazolium compound by living cells, which produces a colored formazan product soluble in cell culture media. This reaction is believed to be mediated by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The amount of formazan dye generated by viable cells can be measured by assessing the absorbance at 490-500 nm. The Cell Meter™ Colorimetric MTS Cell Proliferation Kit is useful for evaluating cell proliferation in response to various stimuli such as growth factors, cytokines, mitogens, and nutrients. It is also applicable for analyzing the effects of cytotoxic compounds, including anticancer drugs and other toxic agents and pharmaceutical compounds. A key advantage of this kit is its ease of use as it can be applied directly to cell culture media (ready-to-use), bypassing the intermediate steps required by traditional MTT assays. Furthermore, this high-throughput assay simplifies the process by eliminating the need for washing or solubilization steps and is suitable for use in 96-well microtiter plates.

References

View all 71 references: Citation Explorer

Assessing Acanthamoeba cytotoxicity: comparison of common cell viability assays.

Authors:

Loufouma Mbouaka, Alvie and Lesiak-Markowicz, Iwona and Heredero-Bermejo, Irene and Mazumdar, Rounik and Walochnik, Julia and Martín-Pérez, Tania

Journal:

Frontiers in microbiology (2023): 1175469

Evaluation of the chemical, physical, and biological properties of a newly developed bioceramic cement derived from cockle shells: an in vitro study.

Authors:

Wannakajeepiboon, Monthip and Sathorn, Chankhrit and Kornsuthisopon, Chatvadee and Santiwong, Busayarat and Wasanapiarnpong, Thanakorn and Linsuwanont, Pairoj

Journal:

BMC oral health (2023): 354

Effect of exposure conditions on chemical properties of materials for surgical endodontic procedures.

Authors:

Koutroulis, Andreas and Valen, Håkon and Ørstavik, Dag and Kapralos, Vasileios and Camilleri, Josette and Sunde, Pia Titterud

Journal:

European journal of oral sciences (2023): e12943

Methotrexate loaded in alginate beads for controlled drug release against breast cancer.

Authors:

Taran, Zahra and Yektaniroumand Digehsaraei, Sepideh and Salouti, Mojtaba and Amini, Bahram and Mahmazi, Sanaz and Kalantari, Mohsen

Journal:

Gene (2023): 146941

Enhanced chemotherapeutic efficacy of docetaxel in human lung cancer cell line via GLUT1 inhibitor.

Authors:

Bahremani, Mona and Rashtchizadeh, Nadereh and Sabzichi, Mehdi and Vatankhah, Amir Mansour and Danaiyan, Sepideh and Poursistany, Haniyeh and Mohammadian, Jamal and Ghorbanihaghjo, Amir

Journal:

Journal of biochemical and molecular toxicology (2023): e23348

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	490 nm
Recommended plate	Clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 1, 2025