Cell Meter™ Colorimetric MTS Cell Proliferation Kit

1. Prepare the cells in a clear-bottom, 96-well plate (100 µL/well).

2. Add 20 µL of the MTS Solution to each well.

3. Incubate at 37°C for 1 - 4 hours.

4. Monitor absorbance at OD = 460 nm.

For guidelines on cell sample preparation, please visit:

https://www.aatbio.com/resources/guides/cell-sample-preparation.html

1. Plate 5,000 to 10,000 cells/well in a tissue culture microplate with a clear bottom.

2. Add the test compounds to the cells and incubate for a desired period of time (such as 24, 48, or 96 hours) in a 37°C, 5% CO2 incubator. For blank wells (medium without the cells), add the same amount of test compounds. The suggested total volume is 100 µL for a 96-well plate or 50 µL for a 384-well plate.

   Note: Each cell line should be evaluated on an individual basis to determine the optimal cell density for proliferation or cytotoxicity induction. For proliferation assays, use fewer cells; for cytotoxicity assays, use more cells to start with.

3. Add 20 µL/well (96-well plate) or 10 µL/well (384-well plate) of the MTS Solution to each well.

4. Incubate the plate at 37°C for 1 - 4 hours, protect from light.

   Note: The incubation time could be from 30 minutes to overnight depending on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.

5. Monitor the absorbance increase with an absorbance plate reader at OD = 490 nm.

1. Use a clear-bottomed tissue culture microplate to prepare cell culture. The suggested total volume is 100 µL for a 96-well plate or 50 µL for a 384-well plate.

   Note: We used serially diluted HeLa and Jurkat cell suspension in a clear bottom 96-well plate for the assay

2. Add 20 µL/well (96-well plate) or 10 µL/well (384-well plate) of the MTS Solution to each well.

3. Incubate the plate at 37°C for 1 - 4 hours, protect from light.

   Note: The incubation time could be from 30 minutes to overnight depending on the individual cell type and cell concentration used. Optimize the incubation time for each experiment.

4. Monitor the absorbance increase with an absorbance plate reader at OD =490 nm.