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Cell Meter™ Direct Cell Proliferation Assay Kit
Product key features
Cell Meter™ Direct Cell Proliferation Assay Kit is a DNA-based fluorescence assay optimized for high-throughput assessment of cell proliferation and cytotoxicity.
- Non-metabolic readout: Measures cell proliferation independent of metabolic activity for consistent results across conditions.
- Streamlined work flow: Simple format, eliminates the need for additional washes, cell lysis, or temperature equilibration.
- Sensitive and quantitative: Accurately quantifies cell number based on DNA content with a broad dynamic range.
Product description
Cell Meter™ Direct Cell Proliferation Assay Kit offers an easy solution for quantifying cell proliferation and cytotoxicity in live cell populations. The assay is based on a proprietary cell-permeant DNA-binding dye paired with a signal enhancer that prevents dead-cell staining, ensuring that only intact, live cells are measured. This kit enables sensitive detection in the broad range of 50 to 25000 cells per well and is suitable for high-throughput screening applications.
This kit is compatible with standard fluorescence plate readers and multiplex assay formats making it a versatile choice for a wide range of applications including drug discovery, compound screening and bioproduction monitoring. Cell Meter™ Direct Cell Proliferation Assay Kit delivers accurate, reproducible, and easy-to-interpret data for your cell-based studies without the complexity of traditional assays.
Example protocol
AT A GLANCE
- Prepare the cell samples and treat cells as desired.
- Add dye working solution.
- Incubate for 30 minutes.
- Analyze with fluorescence microplate reader using Ex/Em = 490/525 nm.
Note: Allow all the components to warm to room temperature before opening the vials. Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues.
PREPARATION OF WORKING SOLUTION
Add 12 µL of Nuclear Green LCS1 (Component A) and 24 µL of Signal Enhancer (Component B) to 10 mL of cell culture medium, mix well.
Note: 10 mL volume is sufficient for 100 tests. Make Cell Meter™ Direct dye working solution sufficient for the assays and use promptly.
Note: Store unused Nuclear Green LCS1 (Component A) and Signal Enhancer (Component B) at -20 °C for further use.
SAMPLE EXPERIMENTAL PROTOCOL
The following protocol can be used as a guideline.
- Prepare the cell samples and treat cells as desired. (Cell culture volume = 100 µL/well).
- Add 100 µL of Cell Meter™ Direct dye working solution and incubate for 30 to 60 minutes at 37°C, 5% CO2 incubator, protected from light. (Total volume = 200 µL/well).
- Remove the Cell Meter™ Direct dye working solution and replace it with 100 µL/well of HHBS (Component C).
- Measure the fluorescence using a fluorescence microplate reader with Ex/Em = 490/525 nm (Cutoff = 515 nm).
Spectrum
References
Authors: Shafie, Alaa and Ashour, Amal Adnan
Journal: Journal of inorganic biochemistry (2025): 112909
Authors: Galloway, A and Hofstede, B Ter and Walsh, Alex J
Journal: bioRxiv : the preprint server for biology (2025)
Authors: Zhu, Jianwei and Zhou, Can and Yang, Jian and Wang, Zhenhua
Journal: Molecular imaging and biology (2025): 250-259
Authors: Chen, Long and Xu, Yi and Zhou, Lele and Ma, Ding and Zhang, Rong and Liu, Yifan and Mi, Xianqiang
Journal: Microsystems & nanoengineering (2025): 10
Authors: Gaifas, Lorenzo and Kleman, Jean-Philippe and Lacroix, Françoise and Schexnaydre, Erin and Trouve, Jennyfer and Morlot, Cecile and Sandblad, Linda and Gutsche, Irina and Timmins, Joanna
Journal: Proceedings of the National Academy of Sciences of the United States of America (2025): e2425047122
Safety data sheet