Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.92 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
See also: Annexin-V Staining, Flow Cytometry Reagents
Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.92 |
Our Cell Meter™ FITC-Annexin V binding assay kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses the green fluorescent FITC-Annexin V conjugate that specifically binds PS. The FITC-Annexin V conjugate has been demonstrated to selectively bind PS. This assay kit is optimized to monitor cell apoptosis using a flow cytometer with 488 nm laser and 530/30 nm emission filter (FITC channel).
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 530/30 nm filter |
Instrument specification(s) | FITC channel |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells with test compounds (200 µL/sample)
- Add Annexin V-FITC assay solution
- Incubate at room temperature for 30 - 60 minutes
- Analyze cells with a flow cytometer using FL1 channel (Ex/Em = 490/525 nm)
Important notes
Thaw 100X Propidium Iodide (Component C) at room temperature before starting the experiment.
SAMPLE EXPERIMENTAL PROTOCOL
- Treat cells with test compounds for a desired period of time (4 - 6 hours for Jurkat cells treated with staurosporine) to induce apoptosis. Note: Annexin V flow cytometric analysis on adherent cells is not routinely tested since specific membrane damage may occur during cell detachment or harvesting. However, methods for utilizing Annexin V for flow cytometry on adherent cell types have been previously reported by Casiola-Rosen et al. and van Engelend et al.
- Centrifuge the cells to get 2 - 5 × 105 cells/tube.
- Resuspend cells in 200 µL of Assay Buffer (Component B).
- Add 2 µL of Annexin V-FITC (Component A) into the cells.
- Optional: Add 2 µL of 100X Propidium Iodide (Component C) for necrosis cells.
- Incubate at room temperature for 30 to 60 minutes, protected from light.
- Optional: Add 200 to 300 µL of Assay Buffer (Component B) to increase volume before analyzing the cells with a flow cytometer.
- Monitor the fluorescence intensity of Annexin V-FITC using a flow cytometer with FL1 channel (Ex/Em = 490/525 nm). Measure the cell viability using FL2 channel when propidium iodide is added into the cells.
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.92 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 651 | 660 | 700000 | - |
Cell Meter™ PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* | 565 | 574 | 1960000 | 0.82 |
Images

Figure 1. The detection of binding activity of FITC-Annexin V to phosphatidylserine in Jurkat cells using Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit. Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) in a 37ºC, 5% CO2 incubator for 4-5 hours, and then dye loaded for 30 minutes. The fluorescence intensity of FITC-Annexin V was measured with a FACSCalibur (Becton Dickinson, San Jose, CA) flow cytometer using the FL1 channel.
Citations
View all 7 citations: Citation Explorer
Combinatorial effect of diclofenac with piperine and D-limonene on inducing apoptosis and cell cycle arrest of breast cancer cells
Authors: Sankar, Srivarshini and Muthukaliannan, Gothandam Kodiveri and others,
Journal: Asian Pacific Journal of Tropical Biomedicine (2023): 80
Authors: Sankar, Srivarshini and Muthukaliannan, Gothandam Kodiveri and others,
Journal: Asian Pacific Journal of Tropical Biomedicine (2023): 80
EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification
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Journal: PeerJ (2023): e15860
Authors: Meng, Zhi-Yong and Fan, Yu-Chun and Zhang, Chao-Sheng and Zhang, Lin-Li and Wu, Tong and Nong, Min-Yu and Wang, Tian and Chen, Chuang and Jiang, Li-He
Journal: PeerJ (2023): e15860
miR-330-3p alleviates the progression of atherosclerosis by downregulating AQP9
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Journal: Functional \& Integrative Genomics (2023): 1--11
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Journal: Stem Cell Research \& Therapy (2021): 1--14
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Authors: Liao, Zhonghui and Li, Feize and Tang, Yu and Liu, Weihao and Gao, Jing and Lan, Tu and Yang, Jijun and Liao, Jiali and Liu, Ning and Yang, Yuanyou
Journal: Journal of Radioanalytical and Nuclear Chemistry (2021): 527--537
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Journal: Journal of Radioanalytical and Nuclear Chemistry (2021): 527--537
TBRG4 Knockdown Suppresses Proliferation and Growth of Human Osteosarcoma Cell Lines MG63 Through PI3K/Akt Pathway
Authors: Huang, Fei and Liao, Faxue and Ma, Guangwen and Hu, Yong and Zhang, Chi and Xu, Pengfei and Xu, Tangbing and Chang, Jun
Journal: OncoTargets and therapy (2020): 7271
Authors: Huang, Fei and Liao, Faxue and Ma, Guangwen and Hu, Yong and Zhang, Chi and Xu, Pengfei and Xu, Tangbing and Chang, Jun
Journal: OncoTargets and therapy (2020): 7271
Combined Treatments of Magnetic Intra-Lysosomal Hyperthermia with Doxorubicin Promotes Synergistic Anti-Tumoral Activity.
Authors: El, D Hajj Diab and Clerc, P and Serhan, N and Fourmy, D and Gigoux, V
Journal: Nanomaterials (Basel, Switzerland) (2018)
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Journal: Nanomaterials (Basel, Switzerland) (2018)
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