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Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit
Optimized for Flow Cytometry
Our Cell Meter™ FITC-Annexin V binding assay kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses the green fluorescent FITC-Annexin V conjugate that specifically binds PS. The FITC-Annexin V conjugate has been demonstrated to selectively bind PS. This assay kit is optimized to monitor cell apoptosis using a flow cytometer with 488 nm laser and 530/30 nm emission filter (FITC channel).
<p>The detection of binding activity of FITC-Annexin V to phosphatidylserine in Jurkat cells using Cell Meter&trade; FITC-Annexin V Binding Apoptosis Assay Kit. Jurkat cells were treated without (Blue) or with 1 &mu;M staurosporine (Red) in a 37&ordm;C, 5% CO2 incubator for 4-5 hours, and then dye loaded for 30 minutes. The fluorescence intensity of FITC-Annexin V was measured with a FACSCalibur (Becton Dickinson, San Jose, CA) flow cytometer using the FL1 channel.</p>
<p>The detection of binding activity of FITC-Annexin V to phosphatidylserine in Jurkat cells using Cell Meter&trade; FITC-Annexin V Binding Apoptosis Assay Kit. Jurkat cells were treated without (Blue) or with 1 &mu;M staurosporine (Red) in a 37&ordm;C, 5% CO2 incubator for 4-5 hours, and then dye loaded for 30 minutes. The fluorescence intensity of FITC-Annexin V was measured with a FACSCalibur (Becton Dickinson, San Jose, CA) flow cytometer using the FL1 channel.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22839100 Tests
Price
 
Spectral properties
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)491
Emission (nm)516
Quantum yield
0.92
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
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Page updated on October 8, 2024