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Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit
Blue Fluorescence
Cell Meter™ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation. The assay is non-radioactive and rapid. The TUNEL assay uses the terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of DEAC-dUTP at the free 3’-hydroxyl ends of the fragmented DNAs. The resulted DEAC-labeled DNAs are analyzed by fluorescence microscopy (AMC filter set). Its blue emission can be conveniently multiplexed with GFP labelled targets. Direct incorporation of fluorescent DEAC-labeled nucleotides significantly reduces the number of test steps. The kit is optimized to detect apoptosis in fixed cells and formalin-fixed, paraffin-embedded tissue sections.
<strong>Fluorescence&nbsp;</strong><strong>images of TUNEL assay&nbsp;</strong><strong>with&nbsp;&nbsp;</strong><strong>HeLa cells.&nbsp;</strong>&nbsp;&nbsp;<br />HeLa cells were fixed and treated with or without DNAse for 60 mins at 37 &deg;C. The cells were then stained with Cell Meter&trade; Fixed Cell and Tissue TUNEL Apoptosis Assay Kit. DNA strand breaks showed intense fluorescent staining in DNAse treated cells. The signal was acquired with fluorescence microscope using Violet filter set.
<strong>Fluorescence&nbsp;</strong><strong>images of TUNEL assay&nbsp;</strong><strong>with&nbsp;&nbsp;</strong><strong>HeLa cells.&nbsp;</strong>&nbsp;&nbsp;<br />HeLa cells were fixed and treated with or without DNAse for 60 mins at 37 &deg;C. The cells were then stained with Cell Meter&trade; Fixed Cell and Tissue TUNEL Apoptosis Assay Kit. DNA strand breaks showed intense fluorescent staining in DNAse treated cells. The signal was acquired with fluorescence microscope using Violet filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2285725 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.14
Correction factor (280 nm)0.12
Excitation (nm)411
Emission (nm)472
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission525/50 nm filter
Instrument specification(s)Pacific Orange channel
Fluorescence microscope
ExcitationViolet filter set
EmissionViolet filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024