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Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit
Red Fluorescence
Cell Meter™ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation. The assay is non-radioactive and rapid. The TUNEL assay uses the terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of TMR-dUTP at the free 3’-hydroxyl ends of the fragmented DNAs. The resulted TMR-labeled DNAs are analyzed by fluorescence microscopy (Cy3 or TRITC filter set). Its red emission can be conveniently multiplexed with GFP labelled targets. Direct incorporation of fluorescent TMR-labeled nucleotides significantly reduces the number of test steps. The kit is optimized to detect apoptosis in fixed cells and formalin-fixed, paraffin-embedded tissue sections.
<strong>Fluorescence </strong><strong>images of TUNEL assay </strong><strong>with&nbsp; </strong><strong>HeLa cells. </strong>&nbsp;&nbsp;<br /> HeLa cells were fixed and treated with or without DNAse for 60 mins at 37 &deg;C. The cells were then stained with Cell Meter&trade; Fixed Cell and Tissue TUNEL Apoptosis Assay Kit. DNA strand breaks showed intense fluorescent staining in DNAse treated cells. The signal was acquired with fluorescence microscope using a Cy3 filter set.
<strong>Fluorescence </strong><strong>images of TUNEL assay </strong><strong>with&nbsp; </strong><strong>HeLa cells. </strong>&nbsp;&nbsp;<br /> HeLa cells were fixed and treated with or without DNAse for 60 mins at 37 &deg;C. The cells were then stained with Cell Meter&trade; Fixed Cell and Tissue TUNEL Apoptosis Assay Kit. DNA strand breaks showed intense fluorescent staining in DNAse treated cells. The signal was acquired with fluorescence microscope using a Cy3 filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2285325 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.27
Correction factor (280 nm)0.34
Extinction coefficient (cm -1 M -1)
100000
Excitation (nm)544
Emission (nm)570
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission575/26 nm filter
Instrument specification(s)PE channel
Fluorescence microscope
ExcitationCy3 filter set
EmissionCy3 filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024