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Cell Meter™ Flow Cytometric Calcium Assay Kit
Cell Meter™ Flow Cytometric Calcium Assay Kit provides fluorescence-based assays for detecting intracellular calcium mobilization using a flow cytometer. It can be used for kinetic reading or for endpoint reading. After loading the Calbryte™ 520 AM dye into cells of interest, simply wash the cells and add the calcium flux agonist, one can then read the sample via a flow cytometer using kinetic reading mode or endpoint reading mode. Calbryte™ 520 AM can cross cell membrane passively by diffusion. Once inside the cells, the lipophilic blocking groups of Calbryte™ 520 AM are cleaved by esterase, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells expressing GPCR of interest are stimulated with an agonist, the receptor signals the release of intracellular calcium, which significantly increases the fluorescence of Calbryte™ 520. The Cell Meter™ Flow Cytometric Calcium Assay Kit can be used for monitoring cellular calcium flux as well as cell sorting.
<p>The ATP induced intracellular calcium release was measured by Cell Meter&trade; Flow Cytometric Calcium Assay Kit in CHO-K1 cells. Cells were incubated with Calbryte&trade; 520 AM dye for 30 min at 37 &deg;C before 10 &micro;M ATP was added into the cells. The baseline was acquired and the rest of the cells were analyzed after the addition of ATP. The response was measured over time. The analysis was done on NovoCyte&trade; 3000 Flow Cytometer. The arrows on the graph indicate the time (30 sec) between addition of ATP and the actual analysis.</p>
<p>The ATP induced intracellular calcium release was measured by Cell Meter&trade; Flow Cytometric Calcium Assay Kit in CHO-K1 cells. Cells were incubated with Calbryte&trade; 520 AM dye for 30 min at 37 &deg;C before 10 &micro;M ATP was added into the cells. The baseline was acquired and the rest of the cells were analyzed after the addition of ATP. The response was measured over time. The analysis was done on NovoCyte&trade; 3000 Flow Cytometer. The arrows on the graph indicate the time (30 sec) between addition of ATP and the actual analysis.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
36310100 Tests
Price
 
Spectral properties
Excitation (nm)493
Emission (nm)515
Quantum yield
0.75
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
Other instrumentsArrayScan, FDSS, FlexStation, IN Cell Analyzer, NOVOStar, ViewLux
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Page updated on October 4, 2025