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Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. The measurement of mitochondrial dehydrogenases (e.g. LDH) activity is a well-accepted assay to quantify cell numbers and monitor cell viability. This cell viability assay kit provides a fast, simple, accurate and homogeneous assay for the fluorometric detection of viable cells. This assay is based on the observation that blue and non-fluorescent resazurin is reduced to a pink fluorescent dye (resorufin) by accepting the electron from mitochondrial respiratory chain in live cells. The amount of resorufin produced is directly proportional to the number of living cells. The detection sensitivity of cell proliferation and cytotoxicity assays using this kit is higher than other assays such as MTT. Since the kit components are quite stable with minimal cytotoxicity, a longer incubation (such as 24 to 48 hours) is possible. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The characteristics of its high sensitivity (<100 CHO cells), non-radioactive and no-wash method made the kit suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds and adaptable for a wide variety of instrument platforms. The kit provides all the essential components with an optimized assay protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. Using 20 ul of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 1000 assays. Using 10 ul of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 2,000 assays.
CHO-K1 cell number response was measured with Cell Meter&trade; Fluorimetric Cell Cytotoxicity Assay Kit. CHO-K1 cells at 0 to 10,000 cells/well/100 &micro;L were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 20 &micro;L/well of Assay Solution (Component A) for 3 hours at 37&deg;C. The fluorescence intensity was measured at Ex/Em = 540/590 nm (Cutoff = 570 nm) with NOVOstar instrument (BMG Labtech). The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated.
CHO-K1 cell number response was measured with Cell Meter&trade; Fluorimetric Cell Cytotoxicity Assay Kit. CHO-K1 cells at 0 to 10,000 cells/well/100 &micro;L were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 20 &micro;L/well of Assay Solution (Component A) for 3 hours at 37&deg;C. The fluorescence intensity was measured at Ex/Em = 540/590 nm (Cutoff = 570 nm) with NOVOstar instrument (BMG Labtech). The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated.
protocol
Protocol
COA
CatalogSize
Price
Quantity
227811000 Tests
Price
227825000 Tests
Price
 
Spectral properties
Absorbance (nm)570
Extinction coefficient (cm -1 M -1)
65000
1
Excitation (nm)571
Emission (nm)584
Quantum yield
0.75
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 8, 2025