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Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit
Red Fluorescence Optimized for Flow Cytometry
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability and proliferation. There are a variety of parameters that can be used for monitoring cell viability and proliferation. In normal cells, DNA density changes depending on whether the cell is growing, dividing, resting, or performing its ordinary functions. The progression of the cell cycle is controlled by a complex interplay among various cell cycle regulators. These regulators activate transcription factors, which bind to DNA and turn on or off the production of proteins that result in cell division. Any misstep in this regulatory cascade causes abnormal cell proliferation which underlies many pathological conditions, such as tumor formation. Potential applications for live-cell studies are in the determination of cellular DNA content and cell cycle distribution for the detection of variations in growth patterns, for monitoring apoptosis, and for evaluating tumor cell behavior and suppressor gene mechanisms. This particular kit is designed to monitor cell cycle progression and proliferation using Nuclear Red™ CCS1, a cell cycle stain in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red™ CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer (FL2 channel).
<p>DNA profile in growing and nocodazole treated Jurkat cells. Jurkat cells were treated without (A) or with 100 ng/ml Nocodazole (B) in 37 &deg;C, 5% CO2 incubator for 24 hours before fixed with 70% ethanol, dye loaded with Nuclear Red&trade; CCS1 and treated with RNase A were loaded for 30 minutes. The fluorescence intensity of Nuclear Red&trade; CCS1 was measured with ACEA NovoCyte flow cytometer with the channel of PE-Texas Red. In growing Jurkat cells (A), nuclear stained with Nuclear Red&trade; CCS1 shows G1, S, and G2 phases (A). In nocodazole treated G2 arrested cells (B), frequency of G2 cells increased dramatically and G1, S phase frequency decreased significantly.</p>
<p>DNA profile in growing and nocodazole treated Jurkat cells. Jurkat cells were treated without (A) or with 100 ng/ml Nocodazole (B) in 37 &deg;C, 5% CO2 incubator for 24 hours before fixed with 70% ethanol, dye loaded with Nuclear Red&trade; CCS1 and treated with RNase A were loaded for 30 minutes. The fluorescence intensity of Nuclear Red&trade; CCS1 was measured with ACEA NovoCyte flow cytometer with the channel of PE-Texas Red. In growing Jurkat cells (A), nuclear stained with Nuclear Red&trade; CCS1 shows G1, S, and G2 phases (A). In nocodazole treated G2 arrested cells (B), frequency of G2 cells increased dramatically and G1, S phase frequency decreased significantly.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22842100 Tests
Price
 
Spectral properties
Excitation (nm)537
Emission (nm)618
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission610/20 nm filter
Instrument specification(s)PE-Texas Red channel
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Page updated on September 25, 2025