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Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit
Orange Fluorescence Optimized for Microplate Reader
Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in vivo. It has been challenging to detect and understand the role of NO in biological systems. Cell Meter™ Fluorimetric Intracellular Nitric Oxide Assay Kit provides a sensitive tool to monitor intracellular NO level in live cells. Nitrixyte™ probes are developed and used in our kit as an excellent replacement for DAF-2 for the detection and imaging of free NO in cells. Compared to the commonly used DAF-2 probe, Nitrixyte™ probes have better photostability and enhanced cell permeability. This particular kit uses Nitrixyte™ Orange that can react with NO to generate a bright orange fluorescent product that has spectral properties similar to Cy3® and TRITC. Nitrixyte™ Orange can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using the filter set of Cy3® or TRITC. This kit is optimized for fluorescence imaging and microplate reader applications.
Fluorescence images of endogenous nitric oxide (NO) detection in RAW 264.7 macrophage. Cells were incubated with AAT’s Nitrixyte™ Orange (Left) or DAF-2 diacetate (Right) at the same concentration,  then treated with or without 20 µg/mL of lipopolysaccharide (LPS) and 1 mM L-arginine (L-Arg) at 37°C for 16 hours. The fluorescence signals were measured using a fluorescence microscope equipped with a TRITC (Left) or FITC (Right) filter set, respectively.
Fluorescence images of endogenous nitric oxide (NO) detection in RAW 264.7 macrophage. Cells were incubated with AAT’s Nitrixyte™ Orange (Left) or DAF-2 diacetate (Right) at the same concentration,  then treated with or without 20 µg/mL of lipopolysaccharide (LPS) and 1 mM L-arginine (L-Arg) at 37°C for 16 hours. The fluorescence signals were measured using a fluorescence microscope equipped with a TRITC (Left) or FITC (Right) filter set, respectively.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16350200 Tests
Price
 
Spectral properties
Excitation (nm)552
Emission (nm)575
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 8, 2025