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Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit
Red Fluorescence Optimized for Flow Cytometry
Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in vivo. It has been challenging to detect and understand the role of NO in biological systems. Cell Meter™ Fluorimetric Intracellular Nitric Oxide Assay Kit provides a sensitive tool to monitor intracellular NO level in live cells. Nitrixyte™ probes are developed and used in our kit as an excellent replacement for DAF-2 for the detection and imaging of free NO in cells. Compared to the commonly used DAF-2 probe, Nitrixyte™ probes have better photostability and enhanced cell permeability. This particular kit uses Nitrixyte™ Red that can react with NO to generate a bright red fluorescent product that has spectral properties similar to Texas Red®. Nitrixyte™ Red can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using the filter set of Red. This kit is optimized for flow cytometry applications.
Detection of exogenous nitric oxide (NO) in Jurkat cells upon DEA NONOate treatment (NO donor) using Cell Meter™ Fluorimetric Intracellular Nitric Oxide Assay Kit (Cat#16356). Cells were incubated with Nitrixyte™ Red at 37 °C for 30 minutes. Cells were further treated with (Red line) or without (Blue line) 1 mM DEA NONOate at 37 °C for another 30 minutes. The fluorescence signal was monitored at FL4 channel using a flow cytometer (BD FACSCalibur).
Detection of exogenous nitric oxide (NO) in Jurkat cells upon DEA NONOate treatment (NO donor) using Cell Meter™ Fluorimetric Intracellular Nitric Oxide Assay Kit (Cat#16356). Cells were incubated with Nitrixyte™ Red at 37 °C for 30 minutes. Cells were further treated with (Red line) or without (Blue line) 1 mM DEA NONOate at 37 °C for another 30 minutes. The fluorescence signal was monitored at FL4 channel using a flow cytometer (BD FACSCalibur).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16356100 Tests
Price
 
Spectral properties
Excitation (nm)586
Emission (nm)607
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission610/20 nm filter
Instrument specification(s)Texas Red channel
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Page updated on October 15, 2025