Name: Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*
Brand: AAT Bioquest
SKU: 16317
Price: 406 USD
Availability: InStock

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit

Optimized for Flow Cytometry

Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can damage a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Meanwhile, peroxynitrite can also have protective activities in vivo by contributing to host-defense responses against invading pathogens. Therefore, peroxynitrite is an essential biological oxidant involved in a broad range of physiological and pathological processes. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and understand the role of peroxynitrite in biological systems. In order to address this unmet need, AAT Bioquest's Cell Meter™ Fluorimetric Intracellular Peroxynitrite (ONOO-) Assay Kit provides a sensitive tool to monitor ONOO- level in living cells. AAT Bioquest's DAX-J2™ PON Green is developed as an excellent fluorescent probe, which can specifically react with intercellular ONOO' to generate a bright green fluorescent product. This kit is optimized for flow cytometry.

<p>Detection of peroxynitrite in Jurkat cells upon SIN-1 treatment using AAT Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit (Cat#16317). (A) Jurkat cells were co-incubated with DAX-J2™ PON Green and 200 µM SIN-1 in full medium at 37 ºC for 1 hour. (B) Cells were stained with DAX-J2™ PON Green for 1 hour, washed with PBS and then incubated with 200 µM SIN-1 in full medium at 37 ºC for 16 hours. Cells stained with DAX-J2™ PON Green but without SIN-1 treatment were used as a control. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
16317	100 Tests	Price

Citations

View all 10 citations: Citation Explorer

4-Octyl Itaconate Attenuates Cell Proliferation by Cellular Senescence via Glutathione Metabolism Disorders and Mitochondrial Dysfunction in Melanoma

Authors:

Hayashi, Yoshikazu and Saeki, Ayaka and Yoshimoto, Shohei and Yano, Ena and Yasukochi, Atsushi and Kimura, Soi and Utsunomiya, Tomoe and Minami, Kento and Aso, Yuji and Hatakeyama, Yuji and others,

Journal:

Antioxidants \& Redox Signaling (2025)

Copper nitroprusside: An innovative approach for targeted cancer therapy via ROS modulation

Authors:

Asif, Kanwal and Adeel, Muhammad and Rahman, Md Mahbubur and Bartoletti, Michele and Brezar, Simona Kranjc and Cemazar, Maja and Canzonieri, Vincenzo and Rizzolio, Flavio and Caligiuri, Isabella

Journal:

Biomedicine \& Pharmacotherapy (2024): 116017

Cold atmospheric plasma sensitizes head and neck cancer to chemotherapy and immune checkpoint blockade therapy

Authors:

Wang, Yanhong and Mang, Xinyu and Li, Danni and Wang, Zhao and Chen, Yiliang and Cai, Zhenyu and Tan, Fei

Journal:

Redox Biology (2023): 102991

Quantum Molecular Resonance Inhibits NLRP3 Inflammasome/Nitrosative Stress and Promotes M1 to M2 Macrophage Polarization: Potential Therapeutic Effect in Osteoarthritis Model In Vitro

Authors:

Paolucci, Teresa and Pino, Vanessa and Elsallabi, Osama and Gallorini, Marialucia and Pozzato, Gianantonio and Pozzato, Alessandro and Lanuti, Paola and Reis, Victor Machado and Pesce, Mirko and Pantalone, Andrea and others,

Journal:

Antioxidants (2023): 1358

Low level of antioxidant capacity biomarkers but not target overexpression predicts vulnerability to ROS-inducing drugs

Authors:

Samarin, Jana and Fabrowski, Piotr and Kurilov, Roman and Nuskova, Hana and Hummel-Eisenbeiss, Johanna and Pink, Hannelore and Li, Nan and Weru, Vivienn and Alborzinia, Hamed and Yildiz, Umut and others,

Journal:

bioRxiv (2023): 2023--01

References

View all 10 references: Citation Explorer

Imaging of nucleolar RNA in living cells using a highly photostable deep-red fluorescent probe

Authors:

Zhou B, Liu W, Zhang H, Wu J, Liu S, Xu H, Wang P.

Journal:

Biosens Bioelectron (2015): 189

RNA and DNA binding of inert oligonuclear ruthenium(II) complexes in live eukaryotic cells

Authors:

Li X, Gorle AK, Ainsworth TD, Heimann K, Woodward CE, Collins JG, Keene FR.

Journal:

Dalton Trans (2015): 3594

Low molecular weight fluorescent probes with good photostability for imaging RNA-rich nucleolus and RNA in cytoplasm in living cells

Authors:

Song G, Sun Y, Liu Y, Wang X, Chen M, Miao F, Zhang W, Yu X, Jin J.

Journal:

Biomaterials (2014): 2103

Luminescence of [Ru(bpy)2(dppz)]2+ bound to RNA mismatches

Authors:

McConnell AJ, Song H, Barton JK.

Journal:

Inorg Chem (2013): 10131

Co-aggregation of RNA binding proteins in ALS spinal motor neurons: evidence of a common pathogenic mechanism

Authors:

Keller BA, Volkening K, Droppelmann CA, Ang LC, Rademakers R, Strong MJ.

Journal:

Acta Neuropathol (2012): 733

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

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Page updated on October 8, 2025

Component A: DAX-J2™ PON Green	2 vials
Component B: DMSO	1 vial (100 µL/vial)