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Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit
Deep Red Fluorescence
Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways. Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit uses our proprietary ROS Brite™ 670 sensor to quantify ROS in live cells. The cell-permeable and non-fluorescent ROS Brite™ 670 exhibits a strong fluorescence signal upon reaction with ROS. ROS Brite™ 670 sensor is localized in the cytoplasm. The fluorescence signal of ROS Brite™ 670 sensor can be measured by fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry. The Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit provides a sensitive, one-step fluorimetric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format using either a fluorescence microplate reader or a fluorescent microscope with Cy5 filter.
Detection of ROS in HeLa cells with Cell Meter&trade; Fluorimetric Intracellular Total ROS Activity Assay Kit. HeLa cells were seeded overnight at 15,000 cells/90 &micro;L/well in a Costar black wall/clear bottom 96-well plate. The cells were untreated (control) or treated with 1 mM H<sub>2</sub>O<sub>2</sub> or 100 &micro;M tert-butyl hydroperoxide (TBHP) for 30 minutes at 37 &deg;C. The ROS Brite&trade; 670 working solution (100 &micro;L/well) was added and incubated in a 5% CO2, 37 &deg;C incubator for 1 hour. The fluorescence signal were monitored at Ex/Em = 650/675 nm (Cutoff = 665 nm) with bottom read mode using FlexStation (Molecular Devices).
Detection of ROS in HeLa cells with Cell Meter&trade; Fluorimetric Intracellular Total ROS Activity Assay Kit. HeLa cells were seeded overnight at 15,000 cells/90 &micro;L/well in a Costar black wall/clear bottom 96-well plate. The cells were untreated (control) or treated with 1 mM H<sub>2</sub>O<sub>2</sub> or 100 &micro;M tert-butyl hydroperoxide (TBHP) for 30 minutes at 37 &deg;C. The ROS Brite&trade; 670 working solution (100 &micro;L/well) was added and incubated in a 5% CO2, 37 &deg;C incubator for 1 hour. The fluorescence signal were monitored at Ex/Em = 650/675 nm (Cutoff = 665 nm) with bottom read mode using FlexStation (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22903200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
Fluorescence microscope
ExcitationCy5 filter
EmissionCy5 filter
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation650 nm
Emission675 nm
Cutoff665 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 21, 2025