logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Cell Meter™ Fluorimetric Phagocytosis Assay Kit
Enhanced Red Fluorescence
The Cell Meter™ Fluorimetric Phagocytosis Assay Kit enables robust and sensitive detection of phagocytic activity in live cells using a unique pH-sensitive fluorescent conjugate.
  • pH-activated fluorescence detection: Utilizes Protonex™ Red 600-Zymosan conjugate, a pH-responsive, non-fluorescent probe that fluoresces strongly in acidic phagosomes and phagolysosomes
  • Dual-color assay format: Includes a green fluorescent viability dye to simultaneously assess cell viability (green) and phagocytic function (red)
  • Application versatility: Suitable for immunology research, drug screening, and cellular function analysis using microscopy, flow cytometry, or microplate formats
  • Comparable alternative: Offers a dual-fluorescence solution similar to BioLegend’s Phagocytosis Detection Kit, with enhanced red signal and viability readout
Examination of phagocytosis in RAW 264.7 cells using Cell Meter™ Fluorimetric Phagocytosis Assay Kit (Cat# 21234). RAW 264.7 cells were incubated with Cytochalasin D (to inhibit phagocytosis) or LPS (to induce phagocytosis) followed by incubation with Protonex™ Red 600-Zymosan Beads in growth medium for 60 minutes and then stained with CytoTrace™ Green for 30 minutes. The images were acquired using Keyence Fluorescence microscopy.
Examination of phagocytosis in RAW 264.7 cells using Cell Meter™ Fluorimetric Phagocytosis Assay Kit (Cat# 21234). RAW 264.7 cells were incubated with Cytochalasin D (to inhibit phagocytosis) or LPS (to induce phagocytosis) followed by incubation with Protonex™ Red 600-Zymosan Beads in growth medium for 60 minutes and then stained with CytoTrace™ Green for 30 minutes. The images were acquired using Keyence Fluorescence microscopy.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
21234100 Tests
Price
 
Spectral properties
Excitation (nm)576
Emission (nm)597
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Instrument settings
Fluorescence microscope
Recommended plateBlack wall/clear bottom
Instrument specification(s)Texas Red and FITC filters.
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 18, 2025