Name: Cell Meter™ Fluorimetric Phagocytosis Assay Kit *Enhanced Red Fluorescence*
Brand: AAT Bioquest
SKU: 21234
Price: 457 USD
Availability: InStock

Cell Meter™ Fluorimetric Phagocytosis Assay Kit

Enhanced Red Fluorescence

The Cell Meter™ Fluorimetric Phagocytosis Assay Kit enables robust and sensitive detection of phagocytic activity in live cells using a unique pH-sensitive fluorescent conjugate.

pH-activated fluorescence detection: Utilizes Protonex™ Red 600-Zymosan conjugate, a pH-responsive, non-fluorescent probe that fluoresces strongly in acidic phagosomes and phagolysosomes
Dual-color assay format: Includes a green fluorescent viability dye to simultaneously assess cell viability (green) and phagocytic function (red)
Application versatility: Suitable for immunology research, drug screening, and cellular function analysis using microscopy, flow cytometry, or microplate formats
Comparable alternative: Offers a dual-fluorescence solution similar to BioLegend’s Phagocytosis Detection Kit, with enhanced red signal and viability readout

Examination of phagocytosis in RAW 264.7 cells using Cell Meter™ Fluorimetric Phagocytosis Assay Kit (Cat# 21234). RAW 264.7 cells were incubated with Cytochalasin D (to inhibit phagocytosis) or LPS (to induce phagocytosis) followed by incubation with Protonex™ Red 600-Zymosan Beads in growth medium for 60 minutes and then stained with CytoTrace™ Green for 30 minutes. The images were acquired using Keyence Fluorescence microscopy.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21234	100 Tests	Price

Overview

The Cell Meter™ Fluorimetric Phagocytosis Assay Kit (Enhanced Red Fluorescence) is designed to evaluate phagocytic activity in live cells. Phagocytosis is a cellular process in which cells engulf and digest particles, such as pathogens or cellular debris, to maintain homeostasis. It features a ready-to-use suspension of Zymosan particles conjugated to Protonex™ Red 600, a novel pH-sensitive dye. Unlike traditional fluorophores, Protonex™ Red 600 is non-fluorescent at neutral pH, becoming highly fluorescent only upon acidification making it ideal for monitoring internalization and lysosomal fusion during phagocytosis. Once engulfed, the Zymosan conjugates are trafficked into acidic phagosomes/phagolysosomes, where the Protonex™ Red 600 dye is activated. The spectral property of the dye is similar to that of Texas red, allowing for seamless integration into existing Texas red-compatible detection workflows.
The kit also contains a green fluorescent viability dye to enable multiplexed analysis of live cells and phagocytosis within the same assay. This two-color system provides a powerful and versatile tool for studying the dynamics and regulation of phagocytosis in various biological and immunological contexts. Suitable for applications in immunology, drug screening, and cellular function research, the assay is compatible with fluorescence microscopy, flow cytometry, and microplate-based formats.

References

View all 21 references: Citation Explorer

Aerobic Exercise Ameliorates Alzheimer's Disease-Like Pathology by Regulating Hepatic Phagocytosis of Aβ.

Authors:

Wang, Qing and Hu, Feng-Rui and Gou, Xing-Chun and Wang, Shan and Ji, Nai-Chun

Journal:

Frontiers in bioscience (Landmark edition) (2025): 36597

Influence of a Very High-Molecular Weight Fucoidan from Laminaria hyperborea on Age-Related Macular Degeneration-Relevant Pathomechanisms in Ocular Cell Models.

Authors:

Dörschmann, Philipp and Kopplin, Georg and Thalenhorst, Tabea and Seeba, Charlotte and Ullah, Sadia Fida and Srivastava, Vaibhav and Roider, Johann and Klettner, Alexa

Journal:

Marine drugs (2025)

Effects of Fucoidans on Activated Retinal Microglia.

Authors:

Dörschmann, Philipp and Hunger, Florentine and Schroth, Hannah and Chen, Sibei and Kopplin, Georg and Roider, Johann and Klettner, Alexa

Journal:

International journal of molecular sciences (2024)

Comparison of Fucoidans from Saccharina latissima Regarding Age-Related Macular Degeneration Relevant Pathomechanisms in Retinal Pigment Epithelium.

Authors:

Dörschmann, Philipp and Thalenhorst, Tabea and Seeba, Charlotte and Tischhöfer, Marie-Theres and Neupane, Sandesh and Roider, Johann and Alban, Susanne and Klettner, Alexa

Journal:

International journal of molecular sciences (2023)

A Peptide-Fc Opsonin with Pan-Amyloid Reactivity.

Authors:

Foster, James S and Williams, Angela D and Macy, Sallie and Richey, Tina and Stuckey, Alan and Wooliver, Daniel Craig and Koul-Tiwari, Richa and Martin, Emily B and Kennel, Stephen J and Wall, Jonathan S

Journal:

Frontiers in immunology (2017): 1082

Spectral properties

Excitation (nm)	576
Emission (nm)	597

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Texas Red and FITC filters.

Telephone
Fax
Email	sales@aatbio.com
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Bulk request	Inquire
Custom size	Inquire
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 15, 2026

Component A: Protonex™ Red 600 - Zymosan Beads Conjugate	150 µL
Component B: CytoTrace™ Green	1 vial (lyophilized)
Component C: DMSO	100 µL