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Cell Meter™ Generic Fluorimetric Caspase Activity Assay Kit
Green Fluorescence Optimized for Flow Cytometry
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring generic caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) activation in living cells. Caspases activation is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). This kit uses TF2-VAD-FMK as a fluorogenic indicator for most caspase activity. TF2-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated casepase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the green fluorescent reagent is retained within the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. The reagent will start to react with active caspase enzymes within 15 minutes of addition to the media. The kit provides all the essential components with an optimized assay protocol. It is used for the quantification of most activated caspases activities in apoptotic cells, or for screening caspases inhibitors. The green label allows for direct detection of activated caspases in apoptotic cells by flow cytometry.
Detection of caspase activity using Cell Meter™ Generic Fluorometric Caspase Activity Assay Kit in Jurkat cells. TF2-VAD-FMK fluorescence intensity was induced with the addition of camptothecin in Jurkat cells. Jurkat cells were treated without (Blue) or with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with TF2-VAD-FMK for 1 hour. Response was recorded using BD FACSCalibur flow cytomter using FL-1 channel.
Detection of caspase activity using Cell Meter™ Generic Fluorometric Caspase Activity Assay Kit in Jurkat cells. TF2-VAD-FMK fluorescence intensity was induced with the addition of camptothecin in Jurkat cells. Jurkat cells were treated without (Blue) or with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours, and then dye loaded with TF2-VAD-FMK for 1 hour. Response was recorded using BD FACSCalibur flow cytomter using FL-1 channel.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22821100 Tests
Price
 
Spectral properties
Correction factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)
75000
Excitation (nm)503
Emission (nm)525
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 29, 2025