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Cell Meter™ Generic Fluorimetric Caspase Binding Assay Kit
Red Fluorescence Optimized for Flow Cytometry
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring generic caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) activation in living cells. Caspases activation is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). This kit uses TF5-VAD-FMK as a fluorogenic indicator for most caspase activity. TF5-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated casepase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the red fluorescent reagent is retained within the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. The reagent will start to react with active caspase enzymes within 15 minutes of addition to the media. The kit provides all the essential components with an optimized assay protocol. It is used for the quantification of most activated caspases activities in apoptotic cells, or for screening caspases inhibitors. The green label allows for direct detection of activated caspases in apoptotic cells by flow cytometry.
<p>The increase of caspase is measured by Cell Meter&trade; Generic Fluorimetric Caspase Binding Assay Kit (Cat# 22822) in Jurkat Cells treated with Staurosporine.&nbsp; Jurkat cells were treated with or without 1 &micro;M of Staurosporine for 4 hours before TF5-VAD-FMK and Nuclear Green DCS1 were added. <strong>A</strong>. Binding of TF5-VAD-FMK to Caspase was measured by NovoCyte at Ex/Em=646/659. <strong>B</strong>. The staining of Nuclear Green DCS1 and TF5-VAD-FMK were both measured by NovoCyte and shown in a dot plot. SS: Staurosporine</p>
<p>The increase of caspase is measured by Cell Meter&trade; Generic Fluorimetric Caspase Binding Assay Kit (Cat# 22822) in Jurkat Cells treated with Staurosporine.&nbsp; Jurkat cells were treated with or without 1 &micro;M of Staurosporine for 4 hours before TF5-VAD-FMK and Nuclear Green DCS1 were added. <strong>A</strong>. Binding of TF5-VAD-FMK to Caspase was measured by NovoCyte at Ex/Em=646/659. <strong>B</strong>. The staining of Nuclear Green DCS1 and TF5-VAD-FMK were both measured by NovoCyte and shown in a dot plot. SS: Staurosporine</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22822100 Tests
Price
 
Spectral properties
Correction factor (280 nm)0.027
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)649
Emission (nm)663
Quantum yield
0.27
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
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Page updated on September 25, 2025