Name: Cell Meter Glow™ 3D Cell Viability Assay
Brand: AAT Bioquest
SKU: 21604
Availability: InStock

Cell Meter Glow™ 3D Cell Viability Assay

Cell Meter Glow™ 3D Cell Viability Assay is a luminescence-based ATP assay for measuring cell viability in three-dimensional cell culture models.

ATP-based viability detection: Measures intracellular ATP as an indicator of metabolically active viable cells
3D culture compatibility: Designed for viability analysis in spheroids, organoids, and matrix-supported 3D cell culture models
Improved lysis performance: Supports ATP release from dense 3D cell structures for luminescent viability measurement
Stable glow signal: Provides a sustained luminescent readout for flexible microplate-based assay processing

Luminescent response of 3D-cultured HeLa cells measured using the Cell Meter Glow™ 3D Viability Assay Kit. HeLa cells were plated as a 2-fold serial dilution in Greiner LUMITRAC™ 200 96-well microplates and luminescence was measured using a CLARIOstar plate reader (BMG LABTECH) after 15 minutes and 2 hours of incubation. The assay detected as few as 39 cells under the tested conditions. Luminescence increased with cell number and the response curves were fitted using a four-parameter logistic (4PL) model. Signal was retained for at least 2 hours under the assay conditions.

Protocol

COA

Catalog	Size	Price
21604	100 Tests	Price
21605	1000 Tests	Price
21606	10000 Tests	Price

Overview

Cell Meter Glow™ 3D Cell Viability Assay is designed for measuring viable cells in three-dimensional culture systems using a homogeneous luminescent ATP readout. ATP levels reflect the presence of metabolically active cells, and luciferase-based detection converts ATP-dependent activity into a luminescent signal that can be measured with a standard luminescence microplate reader.

The assay format is intended for 3D culture models, including spheroids, organoids, and cells grown in extracellular matrix-supported environments. Its reagent system is formulated to support cell disruption and ATP recovery from dense 3D structures, enabling endpoint viability analysis in microplate workflows. Cell Meter Glow™ 3D Cell Viability Assay is suitable for 3D cell viability analysis, compound response studies, cytotoxicity testing, and high-throughput screening applications.

References

View all 42 references: Citation Explorer

Evaluation of Combined Treatment With Atorvastatin and SREBP2 Inhibitors Against Colorectal Cancer Cells Under Two-dimensional and Three-dimensional Culture Models.

Authors:

Ishii, Kyota and Tauchi, Yuno and Yano, Tomohiro

Journal:

Anticancer research (2026): 2403-2415

Bone Marrow-Derived Macrophages Loaded with Boron Carbide Nanoparticles Targeting the Glioblastoma Microenvironment for Boron Neutron Capture Therapy.

Authors:

Rudawska, Anna and Szczygieł, Agnieszka and Węgierek-Ciura, Katarzyna and Mierzejewska, Jagoda and Kozień, Dawid and Żeliszewska, Paulina and Chaszczewska-Markowska, Monika and Rusiniak, Piotr and Wątor, Katarzyna and Pędzich, Zbigniew and Pajtasz-Piasecka, Elżbieta and Szermer-Olearnik, Bożena

Journal:

International journal of nanomedicine (2026): 604151

Effects of anlotinib hydrochloride on the expression of immunogenic cell death-related molecules in Cal27 tongue cancer cells.

Authors:

Huang, Pengcheng and Zhu, Jitao and Guo, Lijuan and Zhang, Zong Yao and Guan, Li and Yang, Sen

Journal:

Discover oncology (2025): 639

Chemotoxic and phototoxic effects of liposomal co-delivery of green synthesized silver nanoparticles and ZnPcS4 for enhanced photodynamic therapy in MCF-7 breast cancer cells: An in vitro study.

Authors:

Chota, Alexander and Abrahamse, Heidi and George, Blassan P

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2025): 117986

Anti-cancer drug sensitivity testing and preclinical evaluation of the anti-cancer potential of WEE1 inhibitor in triple-negative breast cancer patient-derived organoids and xenograft models.

Authors:

Ryu, Seungyeon and Kim, Hoe Suk and Lee, Sangeun and Yoon, So-Hyun and Baek, Moonjou and Park, A Young and Lee, Han-Byoel and Kim, Ga Yeon and Park, Kyung Hyeun and Jung, Ji-Jung and Han, Yireh and Lee, Dong Woo and Ku, Bosung and Han, Wonshik

Journal:

Breast cancer research : BCR (2025): 113

Physical properties

Solvent

Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Luminescence microplate reader
Excitation	--
Emission	--
Instrument specification(s)	Solid white

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Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 26, 2026