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Cell Meter™ Glucose Uptake Imaging Kit
Red Fluorescence
Glucose metabolism is a primary source of cellular energy and biomaterials for maintaining cell homeostasis. Surplus glucose is stored as glycogen in muscular and hepatic tissues, with subsequent release into the bloodstream as needed. The GLUT family of transporter proteins facilitates the process of glucose uptake under the regulation of multiple mechanisms, including the modulation by hormones and growth factors like insulin. Notably, cancer cells exhibit a marked propensity for increased glucose uptake and metabolic activity, engaging in aerobic glycolysis to sustain their accelerated proliferation rates. Compounds that hinder glucose uptake in cancer cells exhibit discernible anti-cancer effects. The Cell Meter™ Glucose Uptake Imaging Kit provides a simple and direct method for quantifying glucose uptake across diverse cellular contexts. This kit employs Glutite™ Red 670, a cell-permeable fluorescent glucose tracer, enabling precise molecular sensing and bioimaging via GLUT transporters. The robust red fluorescence emitted by Glutite™ Red 670 (Ex/Em = 651/670 nm) correlates proportionally with cellular glucose uptake, offering a valuable means for quantification via both fluorescence microscopy and flow cytometry techniques.
Fluorescence images of Glutite™ Red 670 uptake in differentiated 3T3-L1adipocytes using Cell Meter™ Glucose Uptake Imaging Kit. Differentiated 3T3-L1 cells at 50,000 cells/wells/100 µL were seeded overnight in a 96-well Poly-D-Lysine black wall/clear bottom plate. The cells were pre-treated with insulin for 2 hours before being treated with Glutite™ Red 670 and phloretin for 60 minutes. The images were acquired using a fluorescence microscope with a Cy5 filter set.
Fluorescence images of Glutite™ Red 670 uptake in differentiated 3T3-L1adipocytes using Cell Meter™ Glucose Uptake Imaging Kit. Differentiated 3T3-L1 cells at 50,000 cells/wells/100 µL were seeded overnight in a 96-well Poly-D-Lysine black wall/clear bottom plate. The cells were pre-treated with insulin for 2 hours before being treated with Glutite™ Red 670 and phloretin for 60 minutes. The images were acquired using a fluorescence microscope with a Cy5 filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
23501100 Tests
Price
 
Physical properties
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)
250000
Excitation (nm)651
Emission (nm)670
Quantum yield
0.27
1
, 
0.4
2
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy5 Filter Set
EmissionCy5 Filter Set
Recommended plateBlack wall/clear bottom
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Page updated on October 17, 2025