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Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit
Blue Fluorescence
Hydrogen peroxide is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in many biological events that are linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. The measurement of this reactive species is helpful for determining how oxidative stress modulates various intracellular pathways. This Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit uses our unique OxiVision™ Blue peroxide sensor to quantify hydrogen peroxide in live cells. OxiVision™ Blue peroxide sensor is cell-permeable, and generates blue fluorescence when it reacts with hydrogen peroxide. This kit provides a sensitive tool to monitor hydrogen peroxide level in living cells, and it is optimized to be used for fluorescence microscopy.
Fluorescence images of intracellular hydrogen peroxide in HeLa cells using Cell Meter&trade; Intracellular Fluorimetric Hydrogen Peroxide Assay Kit (Cat# 11504). HeLa cells at 10,000 cells/well/100 &micro;L were seeded overnight in a Costar black wall/clear bottom 96-well plate. 100 &micro;M H<sub>2</sub>O<sub>2</sub>: HeLa cells were stained with OxiVision&trade; Blue peroxide sensor for 30 minutes and treated with 100 &micro;M hydrogen peroxide at 37 &deg;C for 90 minutes. Control: Cells were stained with OxiVision&trade; Blue peroxide sensor but without hydrogen peroxide treatment. The fluorescence signals were measured using fluorescence microscope with a DAPI filter.
Fluorescence images of intracellular hydrogen peroxide in HeLa cells using Cell Meter&trade; Intracellular Fluorimetric Hydrogen Peroxide Assay Kit (Cat# 11504). HeLa cells at 10,000 cells/well/100 &micro;L were seeded overnight in a Costar black wall/clear bottom 96-well plate. 100 &micro;M H<sub>2</sub>O<sub>2</sub>: HeLa cells were stained with OxiVision&trade; Blue peroxide sensor for 30 minutes and treated with 100 &micro;M hydrogen peroxide at 37 &deg;C for 90 minutes. Control: Cells were stained with OxiVision&trade; Blue peroxide sensor but without hydrogen peroxide treatment. The fluorescence signals were measured using fluorescence microscope with a DAPI filter.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11504100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationDAPI filter
EmissionDAPI filter
Recommended plateBlack wall/clear bottom
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Page updated on October 2, 2025