Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit

Blue Fluorescence Optimized for Flow Cytometry

Hydrogen peroxide is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in many biological events that are linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. The measurement of this reactive species is helpful for determining how oxidative stress modulates various intracellular pathways. This Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit uses our unique OxiVision™ Blue peroxide sensor to quantify hydrogen peroxide in live cells. OxiVision™ Blue peroxide sensor is cell-permeable, and generates blue fluorescence when it reacts with hydrogen peroxide. This kit provides a sensitive tool to monitor hydrogen peroxide level in living cells, and it is optimized to be used in flow cytometry.

Detection of hydrogen peroxide in Jurkat cells using Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit (Cat#: 11505). Jurkat cells were stained with OxiVision™ Blue peroxide sensor for 30 minutes and treated with 100 µM hydrogen peroxide at 37 °C for 90 minutes. Cells stained with OxiVision™ Blue peroxide sensor but without hydrogen peroxide treatment were used as control.

Protocol

SDS

Catalog	Size	Price	Quantity
11505	100 Tests	Price

Citations

View all 6 citations: Citation Explorer

Ultrasound-Triggered Azo Free Radicals for Cervical Cancer Immunotherapy

Authors:

Wang, Yumeng and Lv, Bin and Wang, Han and Ren, Tingting and Jiang, Qian and Qu, Xinyu and Ni, Dalong and Qiu, Junjun and Hua, Keqin

Journal:

ACS nano (2024)

Hyaluronan-decorated copper-doxorubicin-anlotinib nanoconjugate for targeted synergistic chemo/chemodynamic/antiangiogenic tritherapy against hepatocellular carcinoma

Authors:

Tan, Gang and Hou, Guanghui and Qian, Junmin and Wang, Yaping and Xu, Weijun and Luo, Wenjuan and Chen, Xiaobing and Suo, Aili

Journal:

Journal of Colloid and Interface Science (2024)

Antiviral Effects of Pyrroloquinoline Quinone through Redox Catalysis To Prevent Coronavirus Infection

Authors:

Ishak, Nur Syafiqah Mohamad and Numaguchi, Tomoe and Ikemoto, Kazuto

Journal:

ACS Omega (2023)

An Oxygen Supply Strategy for Sonodynamic Therapy in Tuberculous Granuloma Lesions Using a Catalase-Loaded Nanoplatform

Authors:

Hu, Can and Qiu, Yan and Guo, Jiajun and Cao, Yuchao and Li, Dairong and Du, Yonghong

Journal:

International Journal of Nanomedicine (2023): 6257--6274

IL-10 production by granulocytes promotes Staphylococcus aureus craniotomy infection

Authors:

Kak, Gunjan and Van Roy, Zachary and Heim, Cortney E and Fallet, Rachel W and Shi, Wen and Roers, Axel and Duan, Bin and Kielian, Tammy

Journal:

Journal of Neuroinflammation (2023): 1--15

References

View all 152 references: Citation Explorer

Comparative effects of alpha-tocopherol and gamma-tocotrienol against hydrogen peroxide induced apoptosis on primary-cultured astrocytes

Authors:

Mazlan M, Sue Mian T, Mat Top G, Zurinah Wan Ngah W.

Journal:

J Neurol Sci (2006): 5

Effect of antisense oligonucleotide against Smac/DIABLO on inhibition of hydrogen peroxide induced myocardial apoptosis of neonatal rats

Authors:

Liang PF, Huang XY, Long JH, Xiao MZ, Yang XH, Zhang PH.

Journal:

Zhonghua Shao Shang Za Zhi (2006): 175

Cardioprotective role of endogenous hydrogen peroxide during ischemia-reperfusion injury in canine coronary microcirculation in vivo

Authors:

Yada T, Shimokawa H, Hiramatsu O, Haruna Y, Morita Y, Kashihara N, Shinozaki Y, Mori H, Goto M, Ogasawara Y, Kajiya F.

Journal:

Am J Physiol Heart Circ Physiol (2006): H1138

Simple and rapid determination of hydrogen peroxide using phosphine-based fluorescent reagents with sodium tungstate dihydrate

Authors:

Onoda M, Uchiyama T, Mawatari K, Kaneko K, Nakagomi K.

Journal:

Anal Sci (2006): 815

Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes

Authors:

Bienert GP, Moller AL, Kristiansen KA, Schulz A, Moller IM, Schjoerring JK, Jahn TP.

Journal:

J Biol Chem. (2006)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	450/40 nm filter
Instrument specification(s)	Pacific Blue channel

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Page updated on July 15, 2026

Component A: OxiVision™ Blue peroxide sensor	1 vial
Component B: DMSO	1 vial (200 µL)