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Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit
Green Fluorescence
Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. It is involved in a number of biological events that have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's syndrome. Perhaps the most intriguing aspect of hydrogen peroxide biology is the recent report that antibodies have the capacity to convert molecular oxygen into hydrogen peroxide to contribute to the normal recognition and destruction processes of the immune system. Measurement of this reactive species will help to determine how oxidative stress modulates varied intracellular pathways. This Cell Meter™ Hydrogen Peroxide Assay Kit uses our unique OxiVision™ Green hydrogen peroxide sensor to quantify hydrogen peroxide in live cells. OxiVision™ Green is cell-permeable, and generates the green fluorescence when it reacts with hydrogen peroxide. The kit is an optimized 'mix and read' assay format that is compatible with HTS liquid handling instruments.
Fluorescence images of intracellular hydrogen peroxide in HeLa cells using Cell Meter&trade; Intracellular Fluorimetric Hydrogen Peroxide Assay Kit (Cat#11503). HeLa cells were treated with (left) or without (right) 100 &micro;M hydrogen peroxide at 37<sup>o</sup>C for 90 minutes.
Fluorescence images of intracellular hydrogen peroxide in HeLa cells using Cell Meter&trade; Intracellular Fluorimetric Hydrogen Peroxide Assay Kit (Cat#11503). HeLa cells were treated with (left) or without (right) 100 &micro;M hydrogen peroxide at 37<sup>o</sup>C for 90 minutes.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11503200 Tests
Price
 
Spectral properties
Excitation (nm)498
Emission (nm)517
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationFITC channel
EmissionFITC channel
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 8, 2025