Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation*

Cell Meter™ Intracellular GSH Assay Kit

Optimized for Flow Cytometry with 405 nm excitation

There are a variety of parameters that can be used for monitoring cell apoptosis. This particular kit is designed to detect cell apoptosis by measuring the decrease in reduced glutathione (GSH). GSH is important for maintaining redox level of cells. It is involved in many cellular processes including the scavenging of free radicals, drug detoxification, cell signaling, and cell proliferation. The decrease in cellular GSH concentration is an early hallmark in the progression of cell death in response to different apoptotic stimuli in many cells. Our Cell Meter™ Intracellular GSH Assay Kit uses our proprietary ThiolTrace™ Violet 500, which becomes strongly fluorescent upon reacting with thiol (including GSH in cells). In normal cells, probe is highly fluorescent, while in apoptotic cells, staining intensity is decreased. Cells stained with ThiolTrace™ Violet can be visualized with a flow cytometer (Pacific Orange filter set). The kit can be used together with other reagents, such as 7-AAD (#17501) for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer.

The decrease in the fluorescence intensity of ThiolTrace™ Violet 500 with the addition of Campothecin in HL-60 cells. HL-60 cells were treated for 6 hours without (Red line) or with 20 μM Campothecin (Green line) in a 37 <sup>o</sup>C, 5% CO<sub>2</sub> incubator, and then stained with ThiolTrace™ Violet 500 for 20 minutes. The fluorescence intensity was measured using ACEA NovoCyte 3000 flow cytometer with Pacific Orange channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22809	100 Tests	Price

Citations

View all 50 citations: Citation Explorer

Glutathione S-transferase Pi 1 is a valuable predictor for cancer drug resistance in esophageal squamous cell carcinoma

Authors:

Ogino, S., Konishi, H., Ichikawa, D., Matsubara, D., Shoda, K., Arita, T., Kosuga, T., Komatsu, S., Shiozaki, A., Okamoto, K., Kishimoto, M., Otsuji, E.

Journal:

Cancer Sci (2019): 795-804

The thioredoxin-1 and glutathione/glutaredoxin-1 systems redundantly fuel murine B-cell development and responses

Authors:

Muri, J., Thut, H., Heer, S., Krueger, C. C., Bornkamm, G. W., Bachmann, M. F., Kopf, M.

Journal:

Eur J Immunol (2019): se name="22809.enl" path="C:\Users\aatbi\Drop

Glutathione transferase Omega 1-1 (GSTO1-1) modulates Akt and MEK1/2 signaling in human neuroblastoma cell SH-SY5Y

Authors:

Saisawang, C., Wongsantichon, J., Robinson, R. C., Ketterman, A. J.

Journal:

Proteins (2019): se name="22809.enl" path="C:\Users\aatbi\Drop

Discriminative Detection of Glutathione in Cell Lysates Based on Oxidase-Like Activity of Magnetic Nanoporous Graphene

Authors:

Zhang, H., Chen, J., Yang, Y., Wang, L., Li, Z., Qiu, H.

Journal:

Anal Chem (2019): 5004-5010

A novel antiviral inhibits Zika virus infection while increasing intracellular glutathione biosynthesis in distinct cell culture models

Authors:

Vasireddi, M., Crum, A., May, H., Katz, D., Hilliard, J.

Journal:

Antiviral Res (2019): 46-52

Spectral properties

Excitation (nm)	415
Emission (nm)	499

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	525/50 nm filter
Instrument specification(s)	Pacific Orange channel

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Page updated on October 13, 2025

Component A: ThiolTrace™ Violet 500	1 vial
Component B: Assay Buffer	1 bottle (100 mL)
Component C: DMSO	1 vial (200 µL)